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Nikon Eclipse E600 - Condenser Aperture Diaphragm

Nikon Eclipse E600
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§
2.
Condenser
aperture
diaphragm
Numerical
Plan
40X
_
Aperture
40x
/:0.75°
RE
©
-WD
22
0.75X0.7~0.8=0.525~0.6
The
aperture
diaphragm
is
important
because
it
is
related
to
the
resolution,
contrast,
depth
of
focus
and
brightness
of
the
optical
image.
Turning
the
condenser
aperture
diaphragm
ring
changes
the
size
of
the
aperture
diaphragm.
As
the
aperture
diaphragm
is
stopped
down,
resolution
and
brightness
are
reduced
while
contrast
and
depth
of
focus
are
increased.
Conversely,
as
the
aperture
diaphragm
is
opened,
resolution
and
brightness
are
increased
while
contrast and
depth
of
focus
are
reduced.
It is
not
possible
to
adjust
one
pair
of
characteristics
without
affecting
the
other.
Generally,
a
satisfactory
image
with
appro-
priate
contrast
can
be
obtained
with
an
aperture
setting
that
is
70%
to
80%
of
the
numerical
aperture
of
the
objective.
The
numerical
aperture
is
indicated
on
the
barrel
of
each objective.
An
indication
of
40x/0.75
means
that
the
magnification
is
40x
and
the
numerical
aperture
is
0.75.
If
the
aperture
diaphragm
is
stopped
down
too
far,
the
resolution
is
reduced;
therefore,
except
when
viewing
a
nearly
transparent
specimen,
we
do
not
recommend
stopping
down
the
aperture
to
less
than
60%
of
the
numerical
aperture
of
the
objective.
Adjusting
the
size
of
the
aperture
diaphragm
according
to
the
condenser
scale
Since
the
condenser
scale
indicates
the
numerical
aperture,
adjust
the
aperture
diaphragm
ring
according
to
the
scale.
(Normally,
the
index
on
the
aperture
diaphragm
ring
should
be
aligned
with
the
scale
line
corresponding
to
70%
to
80%
of
the
numerical
aperture
of
the
objective.)
Adjusting
the size
of
the
aperture
diaphragm
by
using
a
centering
telescope
(sold
separately)
Remove
one
of
the
eyepieces
and,
by
using
a
special
adapter
(sold
separately),
mount
a
centering
telescope
in
its
place.
Turn
the
aperture
diaphragm
ring
to
stop
down
the
aperture
diaphragm
as
far
as
possible.
While
holding
down
the
flange
of
the
telescope,
turn
the
eyepiece
of
the
center-
ing
telescope
and
focus
on
the
aperture
diaphragm.
Turn
the
aperture
diaphragm
ring
to
adjust
the
size
of
the
aperture
diaphragm.
(Normally,
the
aperture
diaphragm
should
be
adjusted
so
that
it
is
about
70%
to
80%
of
the
size
of
the
field
being
viewed.)
Remove
the
centering
telescope
and
adapter,
and
then
re-fit
the
eyepiece.
18

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