Chapter 2 Microscopy Techniques
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13 Change the objective to the objective of the
magnification used for observation.
Rotate the revolving nosepiece to bring the objective
of the desired magnification into the optical path.
Do not directly switch from the 4x objective to
the 60x or 100x objective. Be sure to focus on
the specimen with the 10x objective before
using the objective of a higher magnification.
When using an oil-immersion objective
Apply immersion oil between the specimen and the
objective.
When light intensity management is on
When the light intensity management function is
turned on (LIM), bringing the objective into the
optical path loads the memorized brightness.
Switch to the objective to be used for observation.
14 Adjust the aperture diaphragm according to the
objective.
Check the NA value of the objective, and set the
aperture diaphragm lever to the position of the same
figure as the NA of the objective.
→ Section 3.8, “Adjustment of Aperture Diaphragm”
Adjust the aperture diaphragm.
15 Focus on the specimen.
Rotate the coarse and then fine focus knobs to focus
on the specimen.
Focus on the specimen.
perture diaphragm
lever
Mark
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