Chapter 2 Microscopy Techniques
78
9 Switch to bright-field microscopy.
(1) Darken diascopic illumination, and then turn off
diascopic illumination.
Turn the brightness control knob to darken diascopic
illumination. Then, press the illumination on/off button
to turn off diascopic illumination.
(2) Insert the dark-field slider from the left side up to
the click position, and bring the empty position
into the optical path.
The annular diaphragm is removed from the optical
path, and the microscope is set to bright-field
microscopy.
Move your eyes away the eyepieces during
position switching.
Beware that if you remove the barrier filter
from the optical path while looking into the
eyepiece during dark-field microscopy, bright
light might enter your eyes.
When setting the microscope back to
bright-field microscopy, either turn off
diascopic illumination or keep your eyes away
from the eyepiece.
(3) To continue observation, turn on diascopic
illumination.
Press the illumination on/off button to turn on
diascopic illumination.
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