Nikon Eclipse TE300 - Photomicrography; Checking before Photomicrography; Relationship between Photomask and Photomicrography Range; Focusing in Photomicrography

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Warning/Caution Symbols Used in this Manual and on the Equipment3
Warning4
Caution5
Notes on Handling the Microscope6
Contents7
Nomenclature8
System Components (Right-Hand)8
System Components (Left-Hand)9
System Components (Rear)10
TE-PS100/TE-PSE-100 Power Supply10
Connector Pin Assignments10
Basic Microscopy11
Turn on the Power12
Adjust the Brightness of the Dial12
Set the Optical Path for the Observation12
Select the NCB11 Filter12
Fully Open the Field Aperture and Condenser Aperture Diaphragms13
Adjust the Condenser Position13
Move the 10X Objective into the Optical Path13
Adjust the Eyepiece Diopter14
Adjust the Interpupillary Distance14
Place a Specimen on the Stage14
Focus15
Center the Condenser15
Center the Condenser with the 40X Objective15
Perform Microscopy with a Desired Objective16
Phase Contrast Microscopy17
Switch to the Phase Contrast Objective17
Bring the Phase Annular Diaphragm into the Optical Path17
Center the Annular Diaphragm17
Perform Microscopy with a Desired Phase Contrast Objective18
System Condenser18
ELWD-S Condenser18
Photomicrographic Procedure19
Using a Single-Lens Reflex Camera20
Using a Photomicrographic Equipment20
Turn on the Camera20
Adjust the Lamp Brightness20
Select the Optical Path for Photomicrography21
Set the Filters21
Compose the Frame21
Adjust the Focus21
Adjust the Diaphragm22
Check the Exposure Time22
Prevent External Light from Coming in22
Press the Shutter22
Operations on Components23
On/Off Switching of Power24
Adjusting the Brightness with the Lamp Intensity Control Dial25
Brighness Adjustment with ND Filters25
Changing Optical Paths26
Filters27
Field Aperture Diaphragm27
Condenser Aperture Diaphragm for TE-TL Eyepiece Tube28
Condenser Aperture Diaphragm for TE-TS Eyepiece Tube28
System Condenser Info29
LWD Condenser Lens29
ELWD Condenser Lens29
Objective30
Ph Codes30
Objective with Correction Ring30
Cover Glass Thickness31
Oil Immersion Objective31
Turret (for TE-TL Eyepiece Tube Only)32
Diopter Adjustment32
Adjusting Interpillary Distance33
Photomasks33
Eyepiece Tube34
Height Adjustment (for TE-TL Eyepiece Tube Only)34
Removing and Turning the Eyepiece Tube34
Focusing Device35
Refocusing Stopper36
Handling Condenser Holder37
Condenser Refocusing Stopper (Applicable Only When Using the LWD Condenser Lens)37
Rotating the Condenser Mount37
Removing the Condenser Holder37
Illumination Pillar38
Tilting the Pillar38
Screw Holes for Mounting Various Devices38
Rectangular Mechanical Stage39
Photomicrography40
Checking before Photomicrography40
Relationship between Photomask and Photomicrography Range40
Focusing in Photomicrography40
Precautions on Photomicrography41
When Using High-Powered Objectives (40X or More)41
When Using Low-Powered Objectives (4X to 20X)41
When Using Nikon F-9042
Assembly43
Installing the Base44
Mounting the Revolving Nosepiece44
Installing the Stage44
Installing the Illumination Pillar, the Diascopic Illumination Unit44
Mounting the Condenser Holder45
Setting the 33Mm Filter45
Mounting the Eyepiece Tube45
Mounting the Eyepieces45
Mounting the Condenser46
Mounting the System Condenser46
Mounting the ELWD-S Condenser46
Installing Objectives47
Installing the Lamp and Lamphouse47
Removing the Lamphouse47
Connecting the Power Supply48
Connecting the Control Cable48
Installing a 35 MM SLR Camera48
Installing the Photomicrographic Equipment (Option)49
Troubleshooting50
Optical Troubleshooting50
Operational Troubleshooting52
Electrical Troubleshooting53
Care and Maintenance54
Lens Cleaning54
Cleaning Painted Surfaces54
Periodical Inspection54
Using the TE-PS30 or TE-PSE30 Power Supply in Combination with the 6V-30W Illumination Pillar55
TE-PS30/TE-PSE30 Power Supply55
Mounting the 6V-30W Illumination Pillar55
Mounting the Condenser When Using an ELWD-S Condenser56
When Using a SLWD Condenser56
When Using an HMC Condenser56
Electrical Specifications. External View57
When the Microscope (Model TE300) Is Used Together with the 12V 100W Illumination Pillar (Model TE-DH 100W)57
When the Microscope (Model TE300) Is Used Together with the 6V 30W Illumination Pillar (Model TE-DS 30W)57
Power Supply TE-PS100/TE-PSE100 (Power Supply for the 12V 100W Illumination Pillar)58
Power Supply TE-PS30/TE-PSE30 (Power Supply for the 6V 30W Illumination Pillar)59
Conforming Standards60

Nikon Eclipse TE300 - Photomicrography; Checking before Photomicrography; Relationship between Photomask and Photomicrography Range; Focusing in Photomicrography

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