Chapter 8 Functions and Operations of the Devices
109
• LED brightness control selection switch, on: Assigns the control right to the control box.
• LED brightness control selection switch, off: Assigns the control right to the base.
For details about the control box, see “11.1, P2-CTLB Control Box” and “11.2, P2-CTLA Control Box.”
OCC (oblique coherent contrast) illumination
This device supports OCC illumination so that a transparent sample can be observed at high contrast using diascopic
illumination with a white LED. OCC illumination is an oblique illumination method suitable for observing the phase objects,
where a sample is obliquely illuminated with a coherent illuminating light by shielding a part of the optical path using a
shading plate. With this illumination method, a colorless transparent sample can be observed with relief and contrast.
To adjust the OCC contrast, slide the OCC adjustment knob at the right front of the base back and forth. Adjust until the
optimum observation state is obtained in combination with the selected magnification and phase amount of the sample.
For ordinary bright-field microscopy using diascopic illumination, set the OCC adjustment control to either the farthest or
closest position. Sliding the control gradually forward or backward from that position will cause the OCC effect to increase.
Sliding the control by 46 mm will result in completely shielded status. Since adjustment in two directions is thus possible,
the direction of the OCC contrast can be reversed.
In addition, the control position (knob position) can be reproduced using the adjustment knob with a scale.
Prism sheet
The LED diascopic illumination base contains a prism sheet to reduce shading. When using the 1.6x or 2x objective, push
in the prism sheet IN/OUT lever. When using the 0.5x or 1x objective, pull out the lever.
Usable objectives and recommended zoom magnifications
Objective
Zoom magnification
Bright-field diascopic illumination OCC illumination
P2-SHR Plan Apo 0.5X From 1.3x Contrast in the field of view
is not uniform.
P2-SHR Plan Apo 1X Entire area From 5.0x
P2-SHR Plan Apo 1.6X Entire area From 4.0x
P2-SHR Plan Apo 2X From 2.0x From 3.0x
* Shading may occur in the low zoom magnification range.
Attachable optional devices
The following devices can be attached to this base:
• P-SXY64 XY Stage
• C-SSL DIA Sliding Stage
• P-DF2 LED Dark Field Unit 2
• C-TRS Tilting Stage
• C-LSL2 LED Epi Illuminator 2 (via an SMZ-U Epi Arm)
• C-FDF Flexible Double Arm Fiber Illumination Unit (via a C-FIDH Fiber Holder)
• P2-POL Simple Polarizing Attachment
Increasing contrast of the OCC observation image
For observations of the OCC observation image with higher contrast, remove the filter dropping ring (attached to the base
optical path immediately under the grass plate). The contrast can be increased by removing the filter dropping ring that
contains a diffuser plate to control excess contrast for OCC.
45-mm dia. filter
A 45-mm dia. filter (no thicker than 5 mm) can be dropped onto the filter dropping ring (attached to the base optical path
immediately under the grass plate).
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