Nikon SMZ25 - Nikon Microscope Specific Operations

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Chapter

Specific Operations

This chapter describes how to work with this microscope system in order to observe samples.

For details about using the individual devices, see Chapter 8.

Main Page

Introduction3
Table of Contents4
Safety Precautions7
Label Attached to the Product7
WARNING and CAUTION Symbols9
Warning9
Caution9
Notes on Handling the Product14
Components15
Microscopy System Using Episcopic Illumination16
Names of the Devices and Operation Sections16
Microscopy System Using Diascopic Illumination and Epi-Fluorescence Illumination17
Names of the Devices and Operation Sections17
Buttons on the P2-RC Remote Controller18
Compact System19
Names of the Devices and Operation Sections19
Microscopy Procedures21
Operation Flowchart22
Procedure for Microscopy under Episcopic Illumination23
Procedure for Microscopy under Diascopic Illumination27
Procedure for Epi-Fluorescence Microscopy31
Assembling33
System Configuration34
Device List36
Assembly Procedures38
Assembling the EPI System or DIA + EPI-FL System38
Assembly of the Compact/Epi System and Compact/Dia + EPI-FL System56
Assembly of Other Devices (Optional)62
Maintenance and Storage67
Cleaning68
Cleaning Lenses68
Cleaning Parts Other than the Lens68
Disinfecting the Product69
Storage69
Periodic Inspection (Charged)69
Specifications71
Performance Properties72
Physical Properties78
Safety Principle79
Safety Principle80
Test Principle (Bright-Field Observation)80
1.2 Intended Purpose80
1.3 Intended User80
1.4 Applicable Standards80
Specific Operations81
Turning on the Power82
Adjusting the Brightness84
Switching the Optical Path of the Tube85
Adjusting the Angle of Depression86
Adjusting the Diopter86
Adjusting the Interpupillary Distance87
Focusing on the Sample88
Changing the Magnification90
Image Zooming90
Switching Objectives (Using the P2-RNI2 Intelligent Nosepiece)91
Shifting a Sample92
Sliding the Stage Plate92
Shifting the Stage in the X and y Directions (P-SXY64 XY Stage)92
Using the Sliding Stage92
Using the Aperture Diaphragm93
Using Episcopic Illumination93
Using Diascopic Illumination94
Using Epi-Fluorescence Illumination95
Switching Filter Cubes95
Selecting a Filter Cube96
Important Notes for Epi-Fluorescence Microscopy96
Remote Operation96
Capturing Images97
Procedure for Capturing Images97
Tips on Microscope Settings for Photomicroscopy98
Internally Saved Information99
Functions and Operations of the Devices101
Zooming Bodies102
SMZ18 Zooming Body102
SMZ25 Zooming Body103
Focus Units, Focus Mount106
P2-FU Focus Unit106
P2-MFU Motorized Focus Unit107
P2-FMDN Focus Mount110
Nosepiece, Focus Mount Adapter112
P2-RNI2 Intelligent Nosepiece112
P2-FM Focus Mount Adapter112
Objectives113
Bases, Stands, and Stages114
P2-PB Plain Base114
P2-DBL LED Diascopic Illumination Base115
P2-DBF Fiber Diascopic Illumination Base115
P-PS32 Plain Stand116
P-DSL32 LED Diascopic Illumination Stand117
P-DSF32 Fiber Diascopic Illumination Stand117
P-SXY64 XY Stage117
C-SSL DIA Sliding Stage118
C-TRS Tilting Stage118
Tubes119
P2-TERG 100/ P2-TERG 50 Trinocular Tilting Tube119
P2-TL100 Trinocular Tube L119
Episcopic Illuminator120
P2-CIA Coaxial Epi Illuminator120
C-FDF Flexible Double Arm Fiber Illumination Unit121
P2-FIR Ring Fiber Illumination Unit121
P2-FIRL2 LED Ring Illumination Unit 2121
Diascopic Illuminator122
P2-DBL LED Diascopic Illumination Base122
P2-DBF Fiber Diascopic Illumination Base124
P-DSL32 LED Diascopic Illumination Stand126
P-DSF32 Fiber Diascopic Illumination Stand129
P-DF2 LED Dark Field Unit 2131
P2-POL Simple Polarizing Attachment131
Epi-Fluorescence Attachment132
P2-EFLI Epi Fluorescence Attachment132
P2-EFLM Motorized Epi Fluorescence Attachment134
Remote Control Devices136
P2-RC Remote Controller136
AZ-FSW Foot Switch138
AZ-PCR Photo Release140
Control/Communication Devices and Relay Devices141
P2-CTLB Control Box141
P2-CTLA Control Box143
P2-RLY Relay Box144
P2-RLYC Relay Cable144
Troubleshooting145
Image Viewing146
Operation146
Motorized Devices147

Nikon SMZ25 - Nikon Microscope Specific Operations

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