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BX61WI
5-4 Macro Reflected Light Fluorescence Observation
}The macro reflected light fluorescence observation makes possible bright, low-magnification fluorescence observation by
combining low-magnification fluorescence mirror units and fluorescence objective.
1 Introduction
1. For the low-magnification fluorescence observation, use a low-magnification fluorescence mirror units.
The increased observation beam diameter of the fluorescence mirror units brightens the fluorescence by about 25%.
However, due to the large size of the fluorescence mirror units, they can be mounted only in every other position when the
BX-URA2 or BX-RFA reflected fluorescence illuminator is used (a total of 3 units can be mounted on each illuminator).
2. When performing transmitted light brightfield observation using a low-magnification fluorescence objective (2X or 4X),
also use the U-SC3 or U-UCD8 swinging condenser. If other condenser is combined, it will not be possible to illuminate
the entire field of view.
3. During low-magnification fluorescence observation, objective switching or stage movement, be careful so that the UIS
objective does not interfere with the specimen or culture container.
4. The low-magnification fluorescence objectives have been designed to manifest performances with no-covered dry
specimens to specimens located 5 mm below water surface level.
As a result, with water immersed specimens, the focused positions of these objectives are different from UIS objectives.
5. To enable macro reflected light fluorescence observation, the following modules should be replaced.
Low-magnification fluorescence
mirror units
U-MGFP/XL
U-MGFPA/XL
U-MF/XL
Sliding revolving
nosepiece
U-SLRE
Low-magnification fluorescence objective
XLFluor2X/340
XLFluor4X/340
Water immersion cap
for XL objective
XL-CAP
Condenser
U-SC3
U-UCD8
UIS objectives
UMPlanFl-W series
LUMPlanFl-W series
UPlanApo10X to 60X
UPlanFl10X to 40X
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