Il!I
U5B
of
the
mechanical
s~
Fig
14
Fig
12
o
•
m
~
~
(5
z
II
Fig
13
lAs
seen
through eyepiece tube,
with eyepiece removed}
11
A Tetasaki holdef I is oplJOnally available to hold a Tetasaki plate
Of
a
65mm-dia.
petri dish. while
an
optional
slide glass hoidel' 2
can
be
used
to
hold
a
1"
x
3"
slide glass
Of
a 54mm-dia.
petri
dIsh on
the stage IFig.
121
21
A
96-wel1
micro-titre
plate
Of
a 24-we1l micro-titre
plate
can
be
directly
mounted
on
the
stage. [Fig
121
* The
35
mm-di
•.
petri dish holder @ provided
is
used
to
prevent
a specimen
veu&!
from falling through
the
opening
in
the
plein
stage. (Fig.
121
mAperture iris diaphragm
An
aperture
iris diaphragm buill in
the
illuminator
is
used
to
limit
lhe
angular aPerture of
an
objecllVll in brightfjeld.
and
controllable
10
oblain
optimum
obitctiVll
perforfT\itf"lOll,
depending
upon
focal
IengIh.
contrast
and
r8lOlution. The ope'llng
of
IN!
aperture
ins
diaphragm should
be
matched
to
the
N.A
of
the
objecti..e in u
•.
It
is
often
preferable,
however,
10
Stop
down
lhe
apertUre d
..
·
pl'lfagm
to
about 70% through 80% of
the
ootectlVe
NA
IF'O
131
*
An
unsuined
specimen can be
more
nsily
observed
with
a
stopped-down
aperture
in bright filtld.
If)
Sening
of
the
correction collar
An
Inverted microscope is conveniently arranged
to
observe
weeimem
contained
In
vessels of various
bonom
thicknesses. In
order
10
al;hieve
optimum
obiectiVll performance of
lhe
inverted
micrOSCoPe, the high N.A. objectives are provided with a
correction
collar
<D.
[Fig 14)
The
correction collar
is
effective
with
a veueI
bottom
from
0 to
2mm
thickness.
11
If
the
thickness of
the
vessel
bottom
is
known.
Match
the
CO'rection collar
to
tM
thickness
of
the
vessel
bollom
by
the
collar
scal.
provided
2) "
lhe
thickness of
the
_I
bollom
Is
unknown:
The
OPtimum POSition for
the
correction
collar can
be
obta,....:l
from
the
image resolution.
After
focus
adjustment,
if
a
satisfac·
tory
sharp image is not
obtained,
rotate
1M correction collar to
the
right
and
left
10
lnat
you
can
compare
the
imagll!i
at
both
sides. Reset
lhe
collar
to
tnt
bener
Image, then S1arting from
lhis position, further rOtate
the
collar
to
the
right
and
left until
both
images can
be
Obtained
for
comparison with each Other.
As you repeat Ihis procedure several times,
you
can find besl
position for the correction collar, Refocus after rotaling the correc·
tion collar.
lEI
Filters
...:===:..
__
~
Optimum
selection
of
filters enhances the
elleaive
performance
in
observati(lfl and
photomicrography,
No
Filter
Pu""""
1 InterlerfnO! 19reenl
4S.IF550
Enh¥Fces Pl'lam
CX)l'ltrast
ifn9S.
2
Neutral
d."ity
(gray) 43ND2S-W45 Reduces hght intensity
w,thout
ctFaog
ng spect
....
1
characteristics
of
the
light
3OUrce.
3
Light balancing 4S.LBD-2N
For color photomicrography With daylight film.
,
Heat abtorbing
45·HAo
Absorbs heat waves from Ihe light
KlUrCl
10
pro·
teet
tnt
specimen.
* This
f,lt.r
IS
bu,lt
In
the
CK2. It
IS
recommended to
add.
second heat absorbing
fllt.r
for prolonged
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