I~'
2 Phase Contrast Observation
I
D
z
Q
~
~
w
~
o
Legend comes
atop.
00
Legend comes atop,
Fig 15
@-@
Fig 18
D Pre aration
of
the
philSe slider
Put the li!tlt annuli for
4X
ill
and 40X
<V
objectives inlO the phase
slider.
The
annulus@
for
lOX
is
also
use<!
for 20X objectives. The
annuli for 4X and
lOX
{lOX I objectives are centered by means of
the
centering
lever@,
(Fig. 15)
Mount the light annulus
<V
for 40X objective into the center hole
of
the
phase slider (as
the
ARROW indicates).
fJ
l:'ko~
..
~rti:i~O~O~flJjh~.~p~h~LiIT;'~;:============::::l
Insert
the
phase slider
<D
into
the
slot
in
the condenser
in
such a
manner that its engraved surface with figures faces upward. and
the notched side toward the lamp housing pillar.
Rotate
the aper-
ture iris diaphragm lever
<V
all
the way
to
the
left, and insen the
green filter provided
intO
the filter
mount
(j).
(Fig. 16)
II
Cantratl 0
tha
; t u
1)
Place a specimen on the
st~
and focus
on
it.
Remove one
of
the
eyepieces from
the
observation tube
and
insert
the
centering telescope CT·5
CD
provided into
the
observation
tube. {Fig. 17)
Ascertain
that
objective magnification and annular ring are matched.
Rotate
the
top
lens assembly
of
the
centering telescope
to
bring
the bright ring (light annulus) and
the
dark ring (phase annulus)
into focus.
Operate
the
centering lever of
the
light annulus until
both
annuli
are
concentric and superimposed. (Fig.
181
*
If
a specimen slide or
the
bottom
surface
of
I cufture
ve$Sill
is
not flat. either annulus
is
likely
to
slip off
the
center while
centering the other.
In
such a
caSIl.
repeat centering adjustment
again. This trouble, however, rarefy occurs with hi!tler magnifica·
tions, so
that
the 40X
philSe
annulus needs no centering adjust·
ment.
21
After centering adjustment with
the
lOX
objective, check
the
centration with
the
20X objective.
3}
After centration
is
complete, remove
the
CT.s
and insert
the
eye·
piece back into
the
observation tube.
Now
it
is
ready for phase contrast observation.
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