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6.3 Reverse Pipetting
This mode is generally used for pipetting highly viscous liquids accurately. It aspirates a little
excess liquid than the selected volume, and then dispenses the exact volume of liquid
selected. The rest of the liquid in the tip needs to be discarded.
Steps:
1. Select Reverse Pipetting from the main menu.
7. Select the desired volume using the Up/Down arrow keys.
2. Press OK to finalize the volume.
3. Press the trigger key to aspirate the volume.
4. Press the trigger key to dispense the volume.
• The excess liquid will remain in the tip.
6. Press the trigger key to discard the excess liquid.
7. Press the trigger key to repeat the process, or press the ESC key to exit the mode.
6.4 Multi Dispense
6.4.1 Repeat Dispense
The repeat dispense mode repeatedly dispenses the selected volume for the selected number
of dispenses. For better accuracy, it will aspirate an excess volume, which needs to be
discarded at the end.
Steps:
1. Select Multi Dispense from the main menu.
2. Select Repeat Dispense from the Multi Dispense menu.
3. Select the desired dispense volume using the Up/Down arrow keys.
4. Select the number of Dispensing Steps. The pipette will calculate the maximum number
of steps possible once you select the dispensing volume.
5. Use the Up/Down arrow keys to modify the number of dispensing steps and then press
the OK button.
6. Press the trigger key to aspirate the liquid.
7. Press the trigger key to dispense the liquid.
• The excess liquid will remain in the tip.
8. Press the trigger key to discard the excess liquid.
9. Press the trigger key to repeat the process, or press the ESC key to exit the mode.
6.4.2 Auto Dispense
Like Repeat Dispense above, except with the pipette automatically dispensing the repeated
volume at a preset time interval.
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