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4.0 Dispensing by Volume: How to get started
Select your pump tubing; use #24 or #15 Silicone tubing if you have a 1082 peristaltic pump head.
Select #24 tubing if you intend to dispense aliquots larger than 10 mL, use #15 pump tubing if you
intend to dispense aliquots smaller than 10 mL.
Consult, Volume Dispense Mode: Edit, go to the following display:
After having connected the tubing to the solution reservoir, fill the tubing with solution and expel all
air from the tubing by pressing the “PRIME” key at the LabTec™ front panel. You are now ready to
setup your dispense using the built-in calibration; go to the following display and press “Edit” key.
Select the following parameters for “Edit 1”:
“DISP VOLUME”
enter “50.00 mL”
“TUBING”
choose “24”
“SNIFFLE” enter “0.3”
“SLOW FACTOR”
enter “2.5 mL”
“PUMP RATE”
enter “80%”
“TIME DELAY”
enter “0.1 sec”
“COUNT”
enter “1”
First press “Exit”, then press “Exec” (key “A”), and then “Select” for Exec 1. The following display
will appear:
When you press the “RUN” key, the LabTec™ will dispense one (1) 50.00 mL aliquot.
At this point you may want to check the accuracy of the LabTec™. For this purpose use an electronic
scale with 0.01-gram readability, e.g. Mettler PG5002-S or equivalent. Dispense at least three
50.00 mL aliquots of distilled water, record the weight of each aliquot, and then determine the aver-
age aliquot weight. For example, assume that you obtained the following results:
Trial 1 50.80g
Trial 2 50.50g
Trial 3 51.10g
AV = Average Value: 50.80g
After you have dispensed the third aliquot, press the RE-CAL key, the LabTec™ will show the following
display:
DV: 50.00
AV: 50.00
Incr. Decr. Select
Use the “Incr.” And “Decr.” Keys until AV = 50.80, the average aliquot size, then press “Select”. The
Calibration has now been corrected and will compensate for the inaccuracy.
Edit Prime
VOLUME SET:
50:00 mL
Press RUN When Ready
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