Welcome page 2
User Guide for Jess
Welcome
Congratulations on bringing Jess into your lab! We welcome you as a new user and are excited to be a part
of your work. This user guide will provide you with information on how Simple Western assays work as well
as other useful operating and installation information.
To help you get the most from Jess, we've added some attention phrases to assist you through the user
guide:
NOTE Points out useful information.
IMPORTANT Indicates information necessary for proper operation of Jess.
CAUTION Cautions you about potentially hazardous situations that could result in injury to you or
damage to Jess.
!WARNING! Warns you that serious physical injury can result if the listed precautions aren’t followed.
Simple Western Assays
A Simple Western size assay is an automated Western - no gels, no transfer devices, no blots, no film and no
manual analysis.
Simple Westerns take place in a capillary. Jess automates all steps of the process for you including sample
loading, protein separation, immunoprobing or labeling, washing, detection and data analysis. Up to 25
samples can be processed in a single run.
Jess performs Simple Western size-based assays: chemiluminescence or fluorescence immunoassays, and
intra-assay protein normalization. All you have to do is pipette your samples and immunoreagents into Jess's
pre-filled plate, load the capillary cartridge and press Start.
Imaging a Blot
Jess also images PVDF or nitrocellulose blotting membranes with proteins transferred from SDS-PAGE gels
using traditional Western blotting procedures. All you have to do is expose your membrane to an enhanced
chemiluminescent (ECL) substrate, load it into the blot cartridge and press Start.
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