MagNA Pure Compact Operator’s Manual - Version 1.3
56
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How to Operate the MagNA Pure Compact Instrument
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SAMPLE ORDERING SCREEN 4 appears.
Note: This screen only appears and accepts informa-
tion about Internal Control Tubes if you selected a
protocol with internal controls on SAMPLE ORDE-
RING SCREEN 2. (See Action 8 above). The program
will skip this screen if you selected a protocol with no
internal control.
Note: Pipette the lysed sample into the
Internal Control Tube before you place the
tube in the cartridge. It is essential for this
action to have an Internal Control Tube
inserted!
䉴 Pipet the proper amount of internal control (as
specified on Sample Ordering Screen 2) into one
of the Internal Control Tubes (not provided in kit;
for purchase information, see chapter C).
䉴 Identify the tube by attaching your own barcode label or writing an ID number on the tube with a permanent
marker.
䉴 Put the filled control tube in position 1 on row 2 of the Tube Rack.
䉴 On SAMPLE ORDERING SCREEN 4, touch IC Sample ID field 1. In that field, enter the ID of Control 1
(i.e., the one you just put in the rack) by either scanning the (self-attached) barcode or using the virtual
keyboard to type the ID number. (Touch the Keyboard button to access the keyboard.)
䉴 Repeat the above control placement and identification steps for each control used
(e.g., for Control position 2, 3, …, 8).
Result: As you fill in the "Internal Control ID" field for each control, the corresponding position in the reagent layout
will change from green (active) to dark blue (installed in instrument).
䉴 Insert the Tube Rack with sample and internal controls into the MagNA Pure Compact Instrument.
䉴 Touch the "IC Tubes inserted" confirmation button.
䉴 The Next button will now be active. Touch this button to confirm the information on this screen and go to
the next programming screen.
SAMPLE ORDERING SCREEN 5 appears. While at
this screen, do the following:
䉴 Put the appropriate number of Elution Tubes
(supplied in the purification kit, with barcode
labels) into the Elution Tube Rack.
Note: Elution Tubes will contain the final purified
nucleic acid after it is eluted from the magnetic beads
with elution buffer.
䉴 Either scan the barcode on each elution tube into
one Elution Tube ID field on SAMPLE ORDERING
SCREEN 5 or type the tube ID into the field
manually with the virtual keyboard.
For convenience you may insert all the
tubes in the rack, then scan all their bar-
codes into the ORDERING screen at the
same time (see picture). Alternatively, you
may scan the barcode from each tube first, then insert it into the rack.
Result: As you fill the Elution tube ID field for each elution tube, the corresponding position in the reagent layout will
change from green (active) to dark blue (installed in instrument).
䉴 Insert the filled Elution Tube Rack into the MagNA Pure Compact Instrument.
䉴 Touch the "Elution tubes inserted" confirmation button.
䉴 The Next button will now be active. Touch this button to confirm the information on this screen and go to
the next programming screen.
Action Handling Software programming
10
11
Performing a Purification Run
Purification
Performing a Purification Run
Purification
57
4_MagNA Pure Compact_ChapterB_2.fm Seite 56 Mittwoch, 8. März 2006 10:13 10
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