8
liquid (2-3 mm) and smoothly release the
operating button up to the starting position.
4. Withdraw the tip from the liquid touching
against the edge of the container to remove
excess.
5. Press the operating button smoothly to the
first stop to deliver the desired volume. Hold
the operating button at the first stop. The
liquid that remains in the tip should not be
included in the delivery.
6. Discard the remaining liquid by pressing the
operating button to the second stop.
9.3. Repetitive reverse technique
1. Follow the reverse technique steps 1 to 5.
2. Continue pipetting by repeating steps 3 to 5
as long as needed.
3. Finally discard the remaining liquid by
pressing the operating button to the second
stop.
10. Recommendations
for good pipetting
- Make sure that the tip is firmly attached to
the tip cone.
- Hold the pipette vertically when aspirating
the liquid and place the tip only a few
millimeters into the liquid.
- Always control the operating button slowly
and smoothly.
- Pre-rinse the tip before aspirating the liquid
by filling and emptying the tip for three to
five times. This is important especially when
pipetting liquids with a viscosity and density
greater than water or liquids with high vapor
pressure (e.g. ethanol).
- Check that the pipette, tip and liquid are at
the same temperature.
- When pipetting liquids with temperatures
different to the ambient temperature change
the tip after each pipetting. Do not pre-rinse
the tip.
- To avoid contamination, do not rest the
pipette on its side especially with the tip
attached.
- Change the tip cone filter regularly
(recommendation after 50 - 250 pipetting
cycles).
- Never strike the tip cone against the tip tray
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