6
8. Operating the pipette
8.1. Setting the volume
The volume of the pipette is clearly shown
through the display. The volume setting is carried
out with the following steps:
1. Press the volume lock and hold it down.
2. Set the volume by rotating the operating
button (clockwise to decrease the volume
and counterclockwise to increase).
3. Release the volume lock.
NOTE: Do not rotate the operating button
without pressing down the volume lock. Do not
rotate the operating button outside the volume
range of the pipette.
8.2. Sealing and ejecting tips
To ensure the maximum accuracy and precision,
Sartorius tips are recommended to be used with
mLINE pipettes. Before fitting a tip ensure that
the pipette tip cone is clean. Press the tip on the
tip cone of the pipette. The pipette is provided
with a spring loaded tip cone for optimal sealing.
Eject the tip by pressing the tip ejector with your
thumb. Make sure that the tip is disposed into a
suitable waste container.
NOTE: If the tip is pressed too hard the tip cone
yields and, as a result, the counter and operating
button moves.
8.3. Protective tip cone filters
The tip cone design of the mLINE pipettes (>10
µl) allows the use of Safe-Cone Filters in the tip
cones. These removable filters prevent liquids
and aerosols from entering the pipette and thus,
protects the pipette from contamination and
damage.
The Safe-Cone Filters are available as Standard
or Plus versions. It is recommended to use the
Standard filter for general applications and the
Plus filter for more demanding applications such
as cell culture, bacterial and virological work and
molecular biology. Filters need to be exchanged
regularly. The interval for filter exchanging is
application dependant but the recommendation is
Tip ejector
+
–
Volume
lock
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