11
Reassembling:
1. Pipettes m3, m10, m20, m100, m200, m1000:
Carefully place the tip cone (3) on the piston
and attach it by screwing the tip cone holder
(2) clockwise.
Pipettes m5000 and m10 ml:
Carefully place the tip cone cylinder (4)
on the piston and screw clockwise. Make
sure that the tip cone (cylinder) is properly
tightened. Avoid over-tightening.
2. Attach the tip ejector collar (1) by screwing it
clockwise.
3. Place the new tip cone filter on its place.
4. Press the operating button several times to
ensure that the grease has spread evenly.
5. Check the performance of the pipette.
NOTE: It is always necessary to check the
performance of the pipette after in-house service
or maintenance.
11.3. Sterilization of the pipette
The entire mLINE pipette can be sterilized by
steam, autoclaving at 121°C, (252°F), 1 atm for
20 minutes. Remove the tip cone filter (if fitted,
Chapter 8.3). The single channel pipettes can be
autoclaved without special preparations. Unscrew
the lower part of the multichannel pipettes by
holding the connecting collar and turning the
lower part 360° counterclockwise. Put the pipette
into the sterilization bag and place it into the
autoclave. After autoclaving, the pipette must
be cooled down and left to dry overnight before
use. Screw the lower part of the multichannel
pipettes by holding the connecting collar and
turning the lower part 360° clockwise. Make
sure that the lower part is properly tightened.
It is recommended to check the performance
of the pipette after every autoclaving. It is also
recommended to grease the piston/seal of the
pipette after every 10 autoclaving.
12. Testing the
performance and
recalibration
It is recommended to check the performance
of your mLINE pipettes regularly (e.g. every 3
months) and always after in-house maintenance.
However, the user should establish a regular
360°
Connecting
collar
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