| 5
Diafiltration|Desalting
1. Set up the system as illustrated in
Diagram 3 for linked modules. If only
one module is required, connect it in
place of the linked modules.
2. Rinse the system as detailed previously.
3. Either place the solution to be purified in
the 500 ml sample|diafiltration reservoir
(prod. no. VFA006), or concentrate a
larger volume to 500 ml or less so that it
is fully contained in the 500 ml reservoir.
Ensure the reservoir lid is firmly closed.
4. Fill a large feed reservoir with exchange
solvent and pump sample through the
system as for concentration.
5. As the volume in the 500 ml reservoir
decreases, the vacuum created draws
exchange solvent through the feed line
from the larger reservoir. Over 99%
solvent exchange can be accomplished
with an exchange volume approximately
5 times the volume of the sample.
Diagram 3: Buffer exchange | diafiltration with Vivaflow 50
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