6
4 Operation
In this section, general instructions for loading and running gels on the HPE
TM
BlueHorizon are described. Running conditions for specific gel types, videos, and
other useful information are provided on the enclosed DVD and on www.serva.de.
Important Information: Always wear powder free disposable gloves when handling
gels or stripes. Do not open a drawer during an active electrophoresis run without
switching off or pause the power supply. Opening a drawer during running power
causes your power supply to detect a “ground leakage”. That may cause a
disturbance of the running programme or in the worst case damages the power
supply.
4.1 Adjust the electrodes
In the electrode lid, the electrode positions can be adjusted to different gels sizes.
When changing the electrode positions, place the electrode lid half way in the park
position and loosen the screws of the first electrode. Be careful to catch the nut on
the underside of the lid into which the screw fits. Then turn the lid and change the
second electrode as described above. Please note: The position for PreCotes is not
fixed but adjustable. Before each run with PreCotes or CleanGels IEF, superpose the
electrodes and the wicks on the gel.
Gels
PreCotes
CleanGel IEF
FocusGel
SDS Gel
CleanGel
2D HPE Triple /
Double Gels
2D HPE Large
Gel
Application
IEF 1D PAGE 2D PAGE
HiRes
2D PAGE
Electrode
position
4.2 Apply a gel
Important Information: To avoid water condensation on the gel surface, do not yet
switch on the chiller.
A specially formulated cooling fluid is added between the surface of the cooling plate
and the gel to ensure good contact, even temperature control and efficient heat
dissipation. For a standard format gel spread 3 ml cooling contact fluid onto the
centre of the cooling plate. For a large format gel spread 6 ml cooling contact fluid
onto the cooling plate in the shape of an “H”:
DOMINIQUE DUTSCHER SAS
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