serva Blue Series - 4.4 Sample preparation and loading

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always hold them horizontal, as holding them at a vertical angle can result in unequal
buffer concentration.
The electrode wicks should overlap the gel by at least 2 mm. It is important that
buffer is not dropped onto the gel surface and therefore avoid moving the buffer
soaked wicks over the gel.
4.3.2 PreCotes, PreNets, CleanGel IEF
Place two electrode wicks (5 mm) soaked with anode and cathode buffer. Apply them
on the corresponding gel edges: acidic solution on the Anode (+) side, basic solution
on the cathode (-) side. Wicks must not extend beyond edge of gel but be aligned
parallel to each other and corresponding to where the electrodes will be placed.
4.3.3 FocusGel
On FocusGels, the electrodes are directly placed onto the gel without the need of
buffer soaked wicks.
4.4 Sample preparation and loading
4.4.1 SDS Gel Kit
1) Add one volume sample to one volume sample buffer (2x) and dilute the sample
to loading concentration with the sample diluter (depends on the sensitivity of
staining method). Reduce and alkylate your sample.
2) Pipette samples into the sample wells.
4.4.2 CleanGel
Prepare samples according to the related gel instruction manual or specific
application procedure.
4.4.3 HPE Gel
1) Equilibrate the IPG strip
SERVA IPG strip equilibrator (Cat. no. HPE-A04) provides a convenient way to
equilibrate IPG strips. After equilibration, the strip can be easily transferred from
the slots holding the first equilibration solution (e.g. DTT) to the slots holding the
second solution (e.g. IAA).
DOMINIQUE DUTSCHER SAS