serva Blue Series - Trouble Shooting; 7.1 Effects during electrophoresis

15
8 Trouble Shooting
Symptom Cause Remedy
7.1 Effects during electrophoresis
No water flow after
chiller is switched
on.
Wrongly connected
tubing or kinked tube.
Straighten tubing. Check tubing:
Chiller-“Out“ connected to chamber-
“In“.
Air bubbles
between film-
backing and cooling
plate.
Insufficient volume of
cool contact fluid
Lift up gel on one side and apply a
higher volume
Excess cooling fluid
around the film
support.
Too much cooling fluid
applied on the cooling
plate.
Remove excess fluid with lint-free
tissue paper.
Water droplets on
gel surface.
Gel was pre-cooled
without lid at high
humidity conditions
leading to water
condensation.
Do not switch on chiller during gel
application and sample loading.
No electric current,
drawer control
lamps do not
illuminate after
starting the power
supply.
Electronic control
detects wrong
orientation of electric
field.
Plug power supply cables in correctly:
black cable to cathode, red cable to
anode.
Electronic control
detects that one or
more draws do not
contain a gel.
When less than four gels are run,
unplug the non-used electrode lids
and place them into the parking
position.
Lids not properly
positioned or not
plugged-in.
Re-position lids and check
connections between lids and
drawers.
Condensation
inside of electrode
lid.
The gel gets hot
during electrophoresis
because of insufficient
heat dissipation.
Do not forget to switch chiller on!
Check chiller temperature and ensure
no other apparatus is connected to
the same chiller.
DOMINIQUE DUTSCHER SAS