16
Front is curved
instead of straight.
The gel gets hot
during electrophoresis
because of insufficient
heat dissipation.
See above
BlueHorizon is subject
to hot exhaust from
chiller or other
apparatus.
Relocate chiller or other apparatus.
The gel gets hot
during electrophoresis
because too much
power is applied per
gel.
If you run less than four gels at
reduce the mA and W settings in the
power supply accordingly. Follow
strictly the manual.
Migration of front is
very slow and will
not reach the anode
in time.
The electric field is too
low.
Adjust the mA and W settings
according to the number of gels run.
Front is slanted, not
straight.
Uneven buffer
concentration within
the electrode wicks.
Always hold electrode wicks
horizontal when carrying them to the
gel.
Condensation water
develops inside
electrode lid near
to IPG strip(s).
Local heat production
at IPG strip(s)
because of
electroendosmotic
effect.
Remove IPG strip(s) from gel after the
first 70 minutes and then continue the
run. Follow strictly the manual.
Minor
disturbance(s) in
the front.
Buffer drop(s) fell on
the gel surface.
Avoid passing wicks over gel surface.
Air bubbles inside the
wicks.
Gently roll wicks in PaperPool to
remove air.
2D Gels: Irregular
bulging of the front
on one side.
Equilibration buffer
unequally distributed
within the IPG strip(s).
Hold the IPG strip(s) horizontal, start
in the middle when placing the strip
into the slot.
DOMINIQUE DUTSCHER SAS
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