serva Blue Series - 7.2 Effects during scanning; 12.4 Effects seen in the result

17
2D Gels: Run stops,
front does not
continue to migrate,
sparking at the IPG
strip(s).
Strong
electroendosmosis
effect at the IPG
strip(s), because it
has not been removed
after the first 70
minutes.
Remove IPG strip(s) from gel after the
first 70 minutes and then continue the
run. Follow strictly the manual.
7.2 Effects during scanning
Gel edges curl up
during scanning.
Gel edges start to dry
out.
Apply Scan-Frame on the edges of
the gel during scanning.
12.4 Effects seen in the result
2D Gels: Horizontal
streaking
The first dimension
IEF separation in the
IPG strip did not work
well because of
inappropriate sample
preparation IEF
separation problems.
Check the trouble shooting guides
supplied by the providers of the IPG
strips and web forums.
2D Gels: Vertical
streaking
Insufficient
equilibration of the
IPG strip.
Use the equilibration buffer supplied
with the HPE gels, weigh-out the
correct amounts of DTT and IAA
(should be of highest reagent quality),
follow the manual.
IPG strip has become
too dry.
Ensure that there is still a thin layer of
buffer on the surface.
Local disturbances
in the pattern
Air bubble in the
cooling contact fluid
layer
Use sufficient cooling contact fluid on
the cooling plate (3 for standard size
gels, or 6 ml for large gels), distribute
is evenly by sliding the gel with the
film-backing several times left and
right.
Air bubble in a buffer
wick
Distribute the buffer solutions evenly
in the wicks by thoroughly rolling
DOMINIQUE DUTSCHER SAS