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Shimadzu Nexera CL Series User Manual

Shimadzu Nexera CL Series
338 pages
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228-97194B
Sep. 2022
Shimadzu Liquid Chromatograph
Nexera CL series
System Guide
Read this manual thoroughly before you use the product.
Keep this manual for future reference.

Table of Contents

Other manuals for Shimadzu Nexera CL Series

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Shimadzu Nexera CL Series Specifications

General IconGeneral
Column OvenYes
SoftwareLabSolutions
Pump TypeBinary Pump
Flow Rate Range0.001 to 10.000 mL/min
Pressure Range0 to 100 MPa
Detector TypesUV-Vis, PDA, Fluorescence, Refractive Index, Conductivity
Column Oven Temperature Range4 to 85 °C

Summary

Introduction

IMPORTANT

Key safety and handling notes for the manual. Ensure manual stays with product, request replacement if lost/damaged.

Notice

Information subject to change, correction of errors, reserved rights, and trademark acknowledgments.

Instruction Manual Components

Lists the documents comprising the instruction manuals for the product.

Indications Used in This Manual

Explains the meaning of precaution symbols and common symbols used in the manual.

Operating Nexera CL System

Describes the intended use, calibration, and quality control of the Nexera CL system.

1 Overview

1.1 System Features

Highlights key intelligence, efficiency, and design advancements of the Nexera CL series.

1.1.1 LC-40D XR CL, LC-40D X3 CL, or LC-40B X3 CL Solvent Delivery Unit

Details the specifications and capabilities of the solvent delivery units.

1.1.2 SIL-40C XR CL or SIL-40C X3 CL Autosampler

Describes the features and capabilities of the autosamplers, including low carryover and throughput.

1.1.3 CTO-40C CL Column Oven

Explains the function and features of the column oven for precise temperature control.

1.1.4 SPD-40 CL UV-VIS Detector

Details the specifications and functions of the UV-VIS detector, including dual wavelength and scanning.

1.2 Optional Parts

Lists and describes available optional parts for system configuration and enhancement.

1.3 Configuration List

Lists compatible units that can be connected to the Shimadzu Nexera CL series.

2 Analysis

2.1 Switching ON the Power Supply and Opening the [Realtime Analysis] Window

Step-by-step guide to powering on the system and launching the LabSolutions analysis software.

2.2 Specifying Analytical Conditions

Instructions on setting up and editing analytical parameters for experiments, including methods.

2.3 Placing Mobile Phases and Autosampler Rinse Solutions

Procedures for preparing and loading mobile phases and rinse solutions into the system.

2.4 Attaching the Column

Steps for physically connecting the analytical column to the column oven.

2.5 Placing Samples

Procedures for preparing and loading sample vials or plates into the autosampler.

2.6 Performing a Single Run Analysis

Detailed steps for executing a single analysis run using a specified method.

2.7 Performing Realtime Batch (Sequence) Analysis

Guide for setting up and running sequential batch analyses for multiple samples.

2.8 Stopping Analysis and Shutting OFF the Power Supply

Procedures for safely stopping analysis and powering down the system components.

2.9 Key Points for Analysis

Important considerations and precautions for optimal analysis performance.

3 Application Operations

3.1 Startup and Shutdown Functions

Explains automated startup and shutdown processes for system readiness and efficiency.

3.2 Startup Function

Details how to automate system startup procedures, including purging and warming.

3.3 Shutdown Function

Explains how to automate system shutdown and cool-down processes.

3.4 Auto-Diagnostics (Pulsation Check) and Auto-Recovery

Describes automatic system checks and recovery mechanisms for solvent delivery problems.

3.5 Autosampler Functions

Covers various pretreatment and injection functions of the autosampler.

3.6 Batch Action Functions

Defines actions to take based on batch analysis test results, like stopping or resuming.

3.7 Using ACTO (Analytical Condition Transfer and Optimization) Functions

Features for transferring and optimizing analytical conditions between systems.

3.8 Useful Data Analysis Functions

Introduces helpful tools for data analysis within the LabSolutions software.

3.9 Column Management

Explains how to manage columns, track usage, and register them for analysis.

4 Hardware Validation

4.1 Hardware Validation Overview

Provides an overview of the hardware validation process and its importance.

4.2 Timing for Hardware Validation

Outlines when hardware validation procedures, including periodic inspections, should be performed.

4.3 Precautions for Periodic Inspections

Details important precautions to follow during periodic hardware inspections.

4.4 Parts Required for Periodic Inspections

Lists the necessary parts, reagents, and supplies for performing hardware validation.

4.5 Periodic Inspection of Solvent Delivery Pumps

Specific inspection procedures for verifying the performance of solvent delivery pumps.

4.6 Periodic Inspection of Autosamplers

Specific inspection procedures for verifying the performance of autosamplers.

4.7 Periodic Inspection of Plate Changers

Specific inspection procedures for verifying the performance of plate changers.

4.8 Periodic Inspection of Column Ovens

Specific inspection procedures for verifying the performance of column ovens.

4.9 Periodic Inspection of UV Absorbance Detectors

Specific inspection procedures for verifying the performance of UV absorbance detectors.

4.10 Periodic Inspections of Systems

Procedures for validating the performance of the entire LC system.

4.11 Corrective Measures if Control Criteria are Not Satisfied

Actions to take if component or system validation criteria are not met.

5 Troubleshooting

5.1 Errors and Operating Problems

General overview of system errors and operating problems encountered during use.

5.2 Resolving Common Troubleshooting Issues

Step-by-step guidance for diagnosing and resolving common system problems.

5.3 Troubleshooting

Detailed troubleshooting based on symptoms, main causes, and corrective actions.

5.4 List of Error Messages

Comprehensive list of error codes, their main causes, and recommended countermeasures.

6 Selecting Components

6.1 Selecting a System

Guidance on choosing appropriate system configurations based on analysis purpose.

6.1.1 System Installation Arrangement

Diagram and list of components for a typical high-pressure gradient system layout.

6.1.2 System Tubing

Details on available tubing kits and their part numbers for system configurations.

6.2 Ultra High-Speed Analysis

Recommendations for selecting components and optimizing parameters for ultra high-speed analysis.

6.3 Selecting a Mixer

Guidance on selecting an appropriate mixer based on system configuration and mobile phase.

6.4 Selecting the Cell

Recommendations for choosing a flow cell based on column type and analysis purpose.

7 Installation

7.1 Installing the System

General instructions for safely installing the entire LC system.

7.1.1 Removing Items Used to Secure the System during Shipping

Steps to remove shipping restraints from system components before installation.

7.1.2 Installing the Autosampler

Instructions for safely and correctly installing the autosampler unit.

7.1.3 Installing the Solvent Delivery Pump (LC-40D XR CL, LC-40D X3 CL, or LC-40B X3 CL)

Procedures for installing the solvent delivery pump module into the system.

7.1.4 Installing the Detectors (SPD-40 CL)

Steps for installing the SPD-40 CL UV-VIS detector onto the system.

7.1.5 Installing the Degassing Unit (DGU-405 CL)

Instructions for installing the degassing unit for mobile phase preparation.

7.1.6 Installing the Reservoir Tray

Steps for installing the reservoir tray to hold solvent bottles.

7.1.7 Installing the Column Oven (CTO-40C CL)

Procedures for installing the column oven module into the system.

7.2 Cable Connections

Instructions for connecting various system cables, including power and optical cables.

7.2.1 Connecting the Power Cord

Steps for safely connecting the power cords to each instrument.

7.2.2 Connecting Optical Cables

Guide for connecting optical communication cables between system components.

7.2.3 Connecting a Degassing Unit (DGU-405 CL) to the Solvent Delivery Pump

Instructions for connecting the degassing unit to the solvent delivery pump.

7.3 Tubing Connections

General guidance and precautions for connecting various tubing lines in the system.

7.3.1 Installing Tubes and Joints for Draining Leakage

Procedures for setting up drainage tubes and waste bottles for leakage management.

7.3.2 Connecting Low-Pressure Flow Channel Tubing

Instructions for connecting low-pressure tubing, including to the degassing unit.

7.3.3 Connecting Tubing for High-Pressure Flow Channels (Connection Methods)

Details on connecting high-pressure flow channels and the types of tubing kits available.

7.3.4 Connecting Tubing for High-Pressure Flow Channels (Gradient Systems)

Specific tubing connection procedures for high-pressure gradient systems.

7.3.5 Connecting Tubing for High-Pressure Flow Channels (Isocratic Systems)

Specific tubing connection procedures for high-pressure isocratic systems.

7.4 Checking Computer and System Controller Connections

Steps for verifying network and system controller connections for computer control.

7.4.1 Setting the Computer IP Address

Guide to configuring the computer's IP address for network connectivity.

7.4.2 Specifying Computer Browser Settings

Instructions for configuring web browser settings for accessing the system controller.

7.4.3 Specifying System Controller Settings

How to configure system controller network and group settings.

7.5 Other Installation Processes

Covers additional installation steps and optional device setup.

7.5.1 Connecting to LabSolutions

Instructions for connecting the system to the LabSolutions software.

7.5.2 Connecting the Computer to the System Controller

Steps for establishing the computer to system controller network connection.

7.5.3 Installing Optional Column Management Functionality (Column Management Device or “CMD”)

Details on installing the optional Column Management Device for column tracking.

8 Addendum

8.1 Mobile Phase Properties

Lists properties of common mobile phases used in LC systems, including physical and chemical characteristics.

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