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Shimadzu Nexera LC-40B X3 CL User Manual

Shimadzu Nexera LC-40B X3 CL
338 pages
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228-97194B
Sep. 2022
Shimadzu Liquid Chromatograph
Nexera CL series
System Guide
Read this manual thoroughly before you use the product.
Keep this manual for future reference.

Table of Contents

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Shimadzu Nexera LC-40B X3 CL Specifications

General IconGeneral
Gradient Composition Accuracy±0.5%
Temperature Stability±0.1 °C
Power Requirements100-240 VAC, 50/60 Hz
Pump TypeBinary or Quaternary Pump
Flow Rate Accuracy±1% or ±1 μL/min, whichever is greater
Injection Volume0.1 to 100 μL (standard)
Injection Precision≤0.25% RSD
Detector TypeMultiple options available (e.g., UV-Vis, PDA, Fluorescence, etc.)
Wavelength Range190 to 900 nm (for UV-Vis/PDA detectors)
Temperature ControlPeltier

Summary

Introduction

IMPORTANT

Key safety and usage guidelines for operating the system and handling manuals.

Operating Nexera CL System

Analysis

Switching ON the Power Supply and Opening the [Realtime Analysis] Window

Step-by-step guide to powering on the system and launching the analysis software.

Specifying Analytical Conditions

Details how to specify and edit analytical parameters for methods.

Performing a Single Run Analysis

Details the procedure for performing a single run analysis using one method file.

Performing Realtime Batch (Sequence) Analysis

Describes how to perform batch analysis using LabSolutions.

Stopping Analysis and Shutting OFF the Power Supply

Details the steps for safely stopping analysis and shutting down the system.

Application Operations

Auto-Diagnostics (Pulsation Check) and Auto-Recovery

Explains auto-diagnostics and auto-recovery functions for instrument errors.

Auto-Recovery

Details the Auto-Recovery function for recovering from solvent delivery problems.

Autosampler Functions

Pretreatment Functions

Describes various pretreatment functions that can be specified for autosamplers.

Using ACTO (Analytical Condition Transfer and Optimization) Functions

Useful Data Analysis Functions

Column Management

Column Registration and Replacement (Discard)

Describes how to register and discard columns using the column manager and CMD.

Hardware Validation

Hardware Validation Overview

Explains the purpose and importance of hardware validation for analytical instruments.

Periodic Inspection of Solvent Delivery Pumps

Core maintenance procedure for solvent delivery pumps.

Periodic Inspection of Autosamplers

Core maintenance procedure for autosamplers.

Periodic Inspection of Column Ovens

Core maintenance procedure for column ovens.

Periodic Inspection of UV Absorbance Detectors

Core maintenance procedure for UV absorbance detectors.

Periodic Inspections of Systems

Checks the performance of the entire system.

Corrective Measures if Control Criteria are Not Satisfied

Provides corrective actions when validation criteria are not met.

Troubleshooting

Errors and Operating Problems

Addresses issues where the system does not function properly or analysis is unsuccessful.

Resolving Common Troubleshooting Issues

Provides solutions for frequently encountered troubleshooting issues.

Solvent Flow Does Not Stabilize

Troubleshooting steps for unstable solvent delivery pressure.

Solvent Delivery Pressure Increases

Diagnoses and resolves issues related to increasing solvent delivery pressure.

Baseline Does Not Stabilize

Troubleshooting steps when the baseline does not stabilize.

Poor Area Value Reproducibility

Addresses issues with unstable area reproducibility in analysis results.

Carryover Does Not Disappear

Troubleshooting steps for eliminating persistent carryover issues.

Symptoms Presumably Due to an Error, Based on the Hardware Status

Infers problem locations based on hardware status symptoms and suggests corrective actions.

Symptoms Presumably Due to an Error, Based on a Chromatogram

Diagnoses errors based on chromatogram symptoms and suggests corrective actions.

List of Error Messages

Comprehensive reference for system error codes, messages, causes, and countermeasures.

System Controller Errors

Lists error codes, messages, causes, and countermeasures for the system controller.

Pump Errors

Details error messages, causes, and countermeasures for the solvent delivery pump.

Autosampler Errors

Lists error codes, messages, causes, and countermeasures for the autosampler.

Column oven Errors

Details error messages, causes, and countermeasures for the column oven.

UV Detector Errors

Lists error codes, messages, causes, and countermeasures for the UV detector.

Installation

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