Dionex AXP/AXP-MS Manual
CAUTION: Be careful not to break the piston when
removing the pump head. Twisting the pump head can
cause the piston to break.
7. Carefully separate the pump head from the pump. Move the pump
head straight out from the pump and remove it from the piston. Be
careful not to break or damage the piston. Also remove the seal
and seal backup washer from the piston if they did not stay in the
pump head. Remove the O-ring.
8. Carefully separate the flush housing from the pump. Move the
flush housing straight out from the pump and remove it from the
piston. Be careful not to break or damage the piston. Also remove
the self-flush diaphragm from the piston by carefully grasping the
sealing flange on two sides and sliding it straight out on the piston,
being careful not to exert lateral pressure that may break the
piston.
5.2.2 Replacing Piston Seals
Lower than normal pressure, pressure variations, and leaks in the
pumping system can all indicate possible problems with the piston
seal. Depending on the fluid or mobile phase used, piston seal
replacement is often necessary after 1000 hours of running time.
The replacement seal kit contains one seal, one backup washer, a seal
insertion/removal tool, a diaphragm, and a pad to clean the piston
when changing the seal.
5.2.2.1 Conditioning New Seals
Note: Use only water or organic solvents to break in new seals. Buffer
solutions and salt solutions should never be used to break in new seals.
Using a restrictor coil or a suitable column, run the pump with a 50:50
solution of isopropanol (or methanol) and water for 30 minutes at 2000
psi of backpressure and a flow rate of 3.00 mL/min.
18
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