Core Modules 33
Light beam interruption
If there is too much litter, material can be used for building a nest and therefore may permanently
interrupts the light beams at this position, i.e. no activity can be measured.
Calorimetry measurements
Prior to start-up, ensure that…
• …the calorimetry system has no leaks.
• …tubing, seals, connections are in proper working condition.
• …the animal cages are in perfect condition (seals and closures in particular).
• …the opening for the temperature sensor in the animal cage cover is closed off if not required.
• …test series are carried out under identical conditions, e.g., temperature, humidity.
• …no errors occur during the measurement as a result of rapid alterations in the 'room climate'
(e.g. opening doors, entering the room, etc.).
• …the temperature in the test area does not cause the animal to have an unwanted excessive
metabolism.
• …the setting of the setting knob "MFC Sample" of the (Master) Control Unit has not been
altered - otherwise a new calibration is necessary.
• …the Air Drying Unit temperature is set correctly.
NOTICE - Instrument damage!
Dusty animal litter may destroy the sensitive measuring and control equipment.
Do NOT use dusty animal litter.
Proceed as follows during start-up:
• Three hours prior to the test, power ON electronic devices.
o Start PC.
o Start software.
o Power ON Control Unit.
• Weigh the animals and insert them into their animal cages.
NOTICE - Instrument damage!
If animals are kept in sealed cages without a supply of fresh air for a long time, they may be
asphyxiated. The first indication of an improper fresh air supply could be condensation on the
cage walls.
• The air pump must be powered ON before inserting animals in cages.
• The system software must be started.
• Set local alarm, see Software Operating Instructions - Calorimetry, Section "Setup Menu,
Calo".
• If available, use Alarm module to allow for remote alarm, see Software Operating Instructions -
Calorimetry, Section "Setup Menu, Calo".
• Close the animal cages.
• Enter the animal weights in the software.
• Depending on the test requirements, provide some habituation time.
• Start measurement. If necessary, exit room and close the door.
• During the measurement, check the procedure at reasonable intervals.
• Remove condensate in the Air Drying Unit system as required.
• After the measurement, remove the animals from the cages first and then only power OFF the
air pump.
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