Zeiss LSM 700 Instructions



InstructionLSM7002017‐12‐07.docx2017‐12‐19


ConfocalLSM700


Tableofcontents

Introduction ...................................................................................................................................... 2
Hardware .......................................................................................................................................... 2
Objectives ...................................................................................................................................... 2
Filterturret .................................................................................................................................... 2
Laserlines ...................................................................................................................................... 3
LightPath ...................................................................................................................................... 3
Startup ............................................................................................................................................. 4
Samplemountingandviewing ......................................................................................................... 5
Software ........................................................................................................................................... 5
Sampleviewing .............................................................................................................................. 6
Acquisition ..................................................................................................................................... 8
LSMChannelmodeconfocalsetting ......................................................................................... 8
Configuration: ........................................................................................................................ 8
Acquisition&parameter
setting ............................................................................................ 10
Singleimageacquisition ........................................................................................................ 12
Multidimensionalacquisition ................................................................................................. 12
Shuttingdown ................................................................................................................................. 17


 

Zeiss LSM 700 Specifications

General

Microscope Type	Confocal Laser Scanning Microscope
Laser Wavelengths	405 nm, 488 nm, 555 nm, 639 nm
Scan Speed	Up to 8 frames per second at 512 x 512 pixels
Objective Magnification	5x to 100x
Software	ZEN
Scanning Method	Galvanometric
Z-Stacking	Yes
Dimensions	Varies depending on configuration
Weight	Varies depending on configuration
Objective Lenses	Various objectives available (air, water, oil immersion)