Inner Workings – Triple Quadrupole MS versus Single Quadrupole MS 2
How Triggered Dynamic MRM works
Agilent 6400 Series Triple Quad LC/MS Concepts Guide 35
Other triggering conditions for each compound
Figure 15 Example of Trigger Window
Trigger Entrance Delay The Trigger Entrance Delay is the number of scans to skip
after the thresholds for the Trigger transitions have been met
within the Trigger Window. If the Trigger Entrance Delay is 2
and the other trigger conditions are met at scan 200, then only
the primaries are acquired at scans 201 and 202 (the next 2
scans). Primary and secondary transitions are acquired starting
at scan 203.
Trigger Delay The Trigger Delay is the number of scans to skip between
acquiring each of the secondary transitions. If the Trigger
Entrance Delay is 0, the Trigger Delay is 1 and the Repeats is
set to 3 and the other trigger conditions are met at scan 200,
then the secondary transitions are acquired at scan 201, scan
203 and scan 205. Only the primary transitions are acquired at
scans 202 and 204. If the Trigger Delay is set to 2 in the
example above, then the secondary transitions are acquired at
scan 201, scan 204, and scan 207. Only the primary transition
are acquired at scans 202, 203, 205 and 206.
Trigger Window The Trigger Window can be a narrower window within the
Peak Retention Time window. The thresholds for the trigger
transitions are only monitored within the Trigger Window. By
default, the Trigger Window is set to 0 which means the
Trigger Window is the same time as the Peak Retention Time
window. The value you enter for the Trigger Window is the full
width of the window. The Trigger Window is from Ret Time -
1/2*(Trigger Window) to Ret Time + 1/2 * (Trigger Window).
Ret Time and Trigger Window are entered in minutes.
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