Installation
32 Cary 100/300/4000/5000/6000i/7000 Spectrophotometers User’s Guide
Aligning the solid sample holder
To align the solid cell holder:
1 Close the sample compartment lid. In the Cary Win UV software,
set the wavelength to 0 nanometers (zero order). Double-click the
Cary WinUV folder on the computer desktop and then double-
click Align. Click the Cary tab and under ‘Instrument
Parameters’, select Zero order. Click Apply.
2 Place a piece of white paper in the light path and note where the
light beam strikes the solid sample slide. The beam should be
centered on the aperture.
3 If the beam is not centered, first align it by moving the slide
holder along the optical rails to the center of the sample
compartment. Tighten the clamping screw on the slide holder.
You will then need to use the 2.5 millimeter hexagonal balldriver
to adjust the three adjusting screws on the optical rail base until
the beam appears to be visually aligned.
The solid sample holder is now aligned and ready for use.
NOTE
You can use a polarizer/depolarizer with the solid sample holder. These are
available from Agilent.
Extra slide holders are also available. Two of these can be screwed
into the ends of the optical rails.
If you want to use liquid rather than solid samples with your Cary
instrument, you will first need to purchase a cell holder base and a
single cell holder from Agilent.
Alternatively, you can buy a variable pathlength cell holder (see
Figure 9) available from Agilent. This will slot into your solid sample
holder base.
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