1200 Series Quarternary Pump User Manual 47
Installing the Pump
3
Priming and Purging the System
Priming with the Pump
When the pumping system has been turned off for a certain time (for example,
overnight) oxygen will rediffuse into the solvent channels between the vacuum
degasser and the pump. Solvents containing volatile ingredients will slightly
lose these, if left in the degasser without flow for a prolonged period of time.
Therefore priming of the vacuum degasser and the pumping system is
required before starting an application.
1 Open the purge valve of your pump (by turning it counterclockwise) and set
flow rate to 3-5 ml/min.
2 Flush the vacuum degasser and all tubes with at least 30 ml of solvent.
3 Set flow to required value of your application and close the purge valve.
4 Pump for approximately 10 minutes before starting your application.
5 Repeat step 1 to step 4 for other solvent channels, where needed.
Tab le 8 Choice of Priming Solvents for Different Purposes
Activity Solvent Comments
After an installation
When switching between
reverse phase and normal
phase (both times)
Isopropanol
Isopropanol
Best solvent to flush air out of
the system
Best solvent to flush air out of
the system
After an installation Ethanol or Methanol Alternative to Isopropanol
(second choice) if no
Isopropanol is available
To clean the system when
using buffers
After a solvent change
Bidistilled water
Bidistilled water
Best solvent to re-dissolve
buffer cristals
Best solvent to re-dissolve
buffer cristals
After the installation of normal
phase seals (P/N 0905-1420)
Hexane + 5% Isopropanol Good wetting properties
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