3 Basic Operation
Operational and assay guidelines for non-37 °C assays
Seahorse XFe Analyzer Operating Manual 29
Operational and assay guidelines for non-37 °C
assays
• For all non-37 °C operation, the XFe Analyzer must equilibrate overnight in
the required ambient temperature.
• If it is required to set up the XFe Analyzer in a cold room, avoid direct fan
sources.
• For all non-37 °C operation, the tray heater must remain ON. Do NOT turn the
tray heater OFF.
• For assay temperatures below 30 °C, hydrate the Sensor Cartridge in the dark
at room temperature.
• Prior to starting an assay, an additional 30 minutes of precalibration
equilibration time has been added to ensure temperature stability.
To adjust the Target Temperature (set point) using the up/down arrows, do the
following:
1 Click the Temperature widget. (See Figure 11.)
The Tray Heater dialog box is displayed. (See Figure 12.)
Figure 11. Temperature widget
Figure 12. Edit temperature settings (Tray Heater dialog box)
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