3 Basic Operation
Performing XF assays
Seahorse XFe Analyzer Operating Manual 25
Performing XF assays
For detailed instructions on using Wave, the software interface for operating the
XFe Analyzer, please see document S7894-10000, Wave User Guide.
Information and protocols for preparing medium associated with XF assays,
experimental design, running XF assays and analyzing XF data may be found
online at www.agilent.com/chem/discoverXF.
XFe status indicator
During an assay, the Status Indicator light on the top of the XFe Analyzer will
change from Blue to Amber if a task requires user interaction or if an error has
occurred, such as:
• To load a Sensor Cartridge or Cell Plate
• To remove a used Sensor Cartridge or Cell Plate
• To accept or cancel an assay if one or more wells did not calibrate properly
after Calibration
• Any errors that can occur during the run, such as barcode read errors for the
Sensor Cartridge, Cell Plate, or a protocol error.
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