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Agilent Technologies Seahorse XFe

Agilent Technologies Seahorse XFe
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Seahorse XFe Analyzer
Operating Manual
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Agilent Technologies Seahorse XFe Specifications

General IconGeneral
ModelSeahorse XFe
Injection Ports4 injection ports per well
MeasurementOxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR)
ApplicationDrug discovery, Toxicology
Cell TypesMammalian
Plate Format96-well microplate
Throughput96 wells
Detection MethodOptical sensors
Temperature ControlYes, 37°C (typical), adjustable
Data OutputReal-time metabolic rate data
Data Analysis SoftwareSeahorse Wave Desktop Software
Software CompatibilityWindows
DimensionsVaries depending on model
WeightVaries by model
Power RequirementsStandard AC power

Summary

Introduction

General Information

Provides an overview of the different models covered by the manual.

Safety Considerations

Details general safety practices and precautions for operating the instrument.

Electrical hazards

Highlights specific electrical hazards and necessary safety measures.

Instrument Overview and Intended Use

Describes the Agilent Seahorse XFe Analyzer's function and its intended applications.

Technical Specifications

Lists the detailed technical specifications for the Seahorse XFe Analyzer models.

Installation

Unpacking and Component Identification

Guides on how to unpack the system and identify all its components.

Installation Procedure

Step-by-step instructions for properly installing the Agilent Seahorse XFe Analyzer.

Suitable locations for the XFe system

Recommendations for optimal laboratory placement of the XFe Analyzer.

Internal components of the XFe Analyzer

Identifies and explains the function of the XFe Analyzer's internal parts.

Setup and interconnects: cable installation

Instructions for connecting cables and setting up communication between instrument components.

Basic Operation

Power and Warm Up

Procedures for powering on the instrument and allowing it to reach operational temperature.

Performing XF assays

Detailed steps for executing XF assays using the Agilent Seahorse XFe Analyzer.

XFe status indicator

Explains the meaning of the status indicator light on the XFe Analyzer.

Wave Controller widgets

Overview of the various control widgets available in the Wave Controller software.

XFe Assays at Non-37 C Temperatures

Guidelines for conducting XFe assays at temperatures other than the standard 37 °C.

Operational and assay guidelines for non-37 C assays

Specific recommendations for operating and performing assays at non-standard temperatures.

Set alarm (temperature tolerance range)

Instructions for configuring temperature alarms and tolerance ranges.

Maintenance

Cleaning and Routine Maintenance

Procedures for cleaning and performing routine maintenance on the XFe Analyzer.

Troubleshooting

Guidance for diagnosing and resolving common issues with the XFe Analyzer.

Barcode errors

Steps to resolve errors related to barcode reading for cartridges and plates.

Contact Information

Provides contact details for technical support and customer service.

Worldwide technical support

Specific contact numbers and email for global technical support.

Ordering

Information on how to order consumables and accessories for the XFe Analyzer.

Additional Resources:

Links to further documentation and online resources for the XFe Analyzer.

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