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injection volume used. The volume is settable in 0.1 µL increments for
the 5 and 10 µL syringes and 1 mL increments for the 100 µL syringe.
The default value is 1 µL irrespective of the configured syringe volume.
This volume will be used with the first injection made. This may be into
either injector 1 or injector 2 depending on the First Injector Used
parameter described below.
Second Injection Volume (0.1 to either 5 or 10 µL, or 1-100 µL syringe
depending on configured syringe) – This selection defines the volume of
sample that will be used for the Second injection. In order to use the
Second Injection Volume you must have the Injector Position parameter
described below set to Both. The Second Injection Volume is ignored if
the Injector Position is set to either 1 or 2. If you have only configured
one injector, then this entry field will not be displayed. The volume is
settable in 0.1 mL increments for the 5 and 10 µL syringes and 1 µL
increments for the 100 µL syringe. The default value is 1 µL irrespective
of the configured syringe volume. This volume will be used with the
second injection made. This may be into either injector 1 or injector 2
depending on the First Injector Used parameter described below.
Use Prep-Ahead? (Yes or No) – This parameter is used to select
whether the Auto Sampler will sample for the next injection immediately
after the current chromatographic run is completed or to cause the Auto
Sampler to sample while the current run is in progress thus improving
throughput. Prep-Ahead works for either liquid or SPME sampling
modes. If you set the Prep-Ahead selection to Yes, the system will use
the Prep-Ahead delay time entered into the method to determine when to
begin sampling for the next run. The Prep-Ahead timer will begin after
the Auto Sampler has completed all of its actions for the current run. For
instance, if you have dual or duplicate injections selected and the second
injection is set to take place 0.5 minutes into the run, the Prep-Ahead
timer for the next sample will start once the second injection is finished.
So if you have set the Prep-Ahead timer to 10 minutes, the system will
actually start sampling at around 10.5 minutes into the run. If your
sample is either heat or light sensitive you may wish to leave the Prep-
Ahead selection at No. Note that the Prep-Ahead timer is independent of
the run time; this allows you to set a next sampling time while the
instrument is either equilibrating or stabilizing if the sample may be heat
or light sensitive. You will have to experiment with sample and solvent
flushes along with any other pre-injection settings for your Auto Sampler
to determine the correct Prep-Ahead time for optimum cycle time and
minimum wait before injection.
Injector Position (1, 2, or Both) – This parameter is used to either limit
the automation to a single injector (either Position 1 or Position 2), or to
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