Prepare Alternative Solution:
Note: Record weights, in grams, of MVS Stock Solution and solvent during
sample preparation.
• Place a clean, amber bottle with cap on the balance pan and press tare.
• Add the amount of MVS Stock Solution determined using the Alternative
Solution Helper instructions above. Replace the cap on the bottle.
• Record the weight once the balance reaches equilibrium.
• Remove the cap, place it on the balance pan and press tare.
• Add the appropriate amount of solvent determined using the Alternative
Solution Helper.
• Replace the cap on the bottle and record the weight of solvent when the
balance has reached equilibrium.
• Mix each Alternative Solution by inversion approximately 20 times.
• Label the bottle with the Stock and Solvent weights, the Solvent Density and
the date.
• Allow the solution to equilibrate to room temperature before using in any tests.
• Add the Alternative Solution to Data Manager.
Alternative Solution Volume Range per Plate Type
Plate Type
Supported
Volume Range
(
µL)
MVS Stock
Solution
96 Well Standard
Profile
0.4 – 9.9
10 – 49.9
1
2
384 Well Standard
Profile
0.1 – 2.49
2.5 – 9.9
1
2
384 Round Well
Low Volume
0.05 – 1.49
1.5 – 3.99
1
2
384 Well Low
Profile
0.04 – 1.49
1.5 – 3.99
1
2
15
Alternative Solution Preparation
(continued)