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HEADSTAGE TUNING PROCEDURE
Axopatch 200B, Copyright 1997-1999, Axon Instruments, Inc.
Set scope to 10 µs/div.
Set FILTER to 100 kHz.
Trim RT29 for fastest rise time without overshoot. The 10 - 90% rise time should be
about 6 - 8 µs.
Set CONFIG. to WHOLE CELL β = 0.1.
Set OUTPUT GAIN to x10
Repeat above steps for β = 0.1, but use RT32 and RT35 for tuning.
Reset Transient Compensation
This is a fairly complicated procedure. The results rely on the patience and skill of the
person who is doing the adjustments. Please confirm that the reset transients are indeed
unacceptable before proceeding. Because of the consistency between units, you may not
have to do these adjustments even if you are changing headstages.
Make sure the unit has been on continually for at least one hour.
Forced Reset
Remove PATCH-1U model cell.
CONFIG. to PATCH.
OUTPUT GAIN to x100.
FILTER to 5 kHz.
Scope to 0.2 V/div, 0.5 ms/div.
Trigger scope from DATA NOT VALID BNC.
Connect pulse generator to FORCED RESET BNC.
Set pulse generator +5 V ( > 100 µs duration), 100 Hz.
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