Home
Bruker
Thermometer
NMR
Bruker NMR User Manual
5
of 1
of 1 rating
40 pages
Give review
Manual
Specs
To Next Page
To Next Page
To Previous Page
To Previous Page
Loading...
Getting Started
12
Z33085_2_002
Figure 4.2:
Edit Solvent Parameters.
In
the
submenu
Lock
window
the
solvents
are
listed,
along
with
the
probe
for
which
the
definition
is
valid
(generic
or
specific
probe),
as
well
as
other
lock
parameters
(lock
power,
lock regulation triplet etc.).
Figure 4.3:
The Submenu Lock.
By
executing
a
right
mouse
click
on
a
solvent
entry,
a
pull-down
menu
opens
with
the
option
for editing the lock parameters:
11
13
Table of Contents
Table of Contents
3
1 Introduction
5
2 Principle of NMR Thermometer
7
Figure 2.1 Principle of the NMR Thermometer
7
3 Requirements
9
4 Getting Started
11
The Edlock Menu
11
Figure 4.1 the Submenu Bar: Solvent
11
Figure 4.2 Edit Solvent Parameters
12
Figure 4.3 the Submenu Lock
12
Setting up the NMR System for the NMR Thermometer
13
Figure 4.4 Edit Lock Parameters
13
Figure 4.5 Edte Window: NMR Thermometer Monitoring Mode (Disabled, Top), Regulation Mode
14
Figure 4.6 Selection of the Auto Phase Algorithm
14
Figure 4.7 Selftune Warning about PID Parameters Misfit
14
Examples
15
Figure 4.8 Starting the Selftune Procedure from the Edte Window
15
Monitoring Mode
16
Figure 4.9 Selftune in Progess
16
Regulation Mode
17
Figure 4.10 Monitoring Several BSMS Values During a Temperature Jump
17
Figure 4.11 Topspin Status Bar with NMR Thermometer Enabled
17
Figure 4.12 Monitoring of the Sensor and the Sample Temperature in Edte During a TOCSY Experiment
18
5 Advanced Operation
19
Define a New Solvent
19
Figure 5.1 Adding New Solvents
19
Figure 5.2 Assign Signals to Type of Signal (Signal, Lock, Temperature)
20
Figure 5.3 Dialog for Importing Temperature Shift Value Files
20
Figure 5.4 Example of a Shift-Temperature File in .Xml Format
20
Selection of NMR Thermometer Compounds
21
Figure 5.5 Temperature Shift Values Filled in
21
Figure 5.6 Suggested Compounds for the NMR Thermometer and Samples in Aqueous Solution
21
Predefined Solvents for the NMR Thermometer
22
Technical Considerations
23
Figure 5.7 Approximate Dynamic Range of Field Lock (Blue) Vs. the NMR Thermometer Signal (Red) if the S/N of the Frequency Lock Signal Is Sufficient (> 200:1)
23
Considerations for Shimming with Topshim
24
Figure 5.8 Typical Concentrations Required for the NMR Thermometer Compound. if the NMR
24
6 Applications
27
Identical Chemical Shifts at Different Spectrometers
27
Figure 6.1 Overlay of a 1H Spectrum of 2 MM Sucrose (Standard Sample) Measured at 600
27
Identical Chemical Shifts for Experiments with Different Heating Due to Experimental Conditions (Temperature Compensation)
28
RF Heating
28
Figure 6.2 Overlay of 15N HSQC Spectra (Overview: Left Part) of 0.5 MM Ubiquitin 9:1 H2O
28
Heating Caused by Spinning Speed (HR-MAS)
29
Figure 6.3 Overlay of NOESY-HSQC (Blue), TOCSY-HSQC (Orange) and CPMG-HSQC (Green) Spectra of 0.5Mm Ubiquitin in 95:5% H2O/D2O Measured at 800Mhz TCI
29
Figure 6.4 1H NMR Spectra on a Liver Sample with Sodium Acetate Added
29
Figure 6.5 Comparison of TOCSY Experiments of a Liver Sample with Sodium Acetate Added Measured with HR-MAS at 4 Khz Spinning Speed
30
7 Frequently Asked Questions (FAQ)
31
8 Contact
33
List of Figures List of Figures
35
List of Figures
35
Index
37
5
Based on 1 rating
Ask a question
Give review
Questions and Answers:
Need help?
Do you have a question about the Bruker NMR and is the answer not in the manual?
Ask a question
Bruker NMR Specifications
General
Brand
Bruker
Model
NMR
Category
Thermometer
Language
English
Related product manuals
Bruker NMR CASE
15 pages
Bruker NMR BSMS System
234 pages
Bruker NMR AVANCE NEO Systems
48 pages
Bruker AVANCE NEO
120 pages
To Next PageTo Next PageTo Previous PageTo Previous Page
Loading...
Need help?
General
