EasyManua.ls Logo

Brunswick BIOFLO 3000 - User Manual

Brunswick BIOFLO 3000
28 pages
Print Icon
To Next Page IconTo Next Page
To Next Page IconTo Next Page
Loading...
BIOFLO 3000
BENCH-TOP
FERMENTOR
MANUAL NO.: M1217-0050/K
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 . 44 TALMADGE ROAD . EDISON, NJ 08818-4005 . 732-287-1200
TELEPHONE: 800-631-5417 . FAX: 732-287-4222 . TELEX: 4753012 NBSCO
INTERNET: http://www.nbsc.com/ . E-MAIL: bioinfo@nbsc.com
M1217-0050 1
Click here to view available Bioflo 3000 Bioreactor at
www.ssllc.com>>

Other manuals for Brunswick BIOFLO 3000

Question and Answer IconNeed help?

Do you have a question about the Brunswick BIOFLO 3000 and is the answer not in the manual?

Summary

Chapter 1 - Introduction

1.1 Scope of Manual

Outlines the manual's content and scope for the BioFlo 3000 system.

1.2 Description of Equipment

Provides an overview of the BioFlo 3000 bioreactor system and its capabilities.

1.3 Description of Vessel

Details the construction and features of the BioFlo 3000's glass and stainless steel vessel.

1.4 Agitation System

Explains the servo motor, speed control, and PID loop for agitation in the BioFlo 3000.

1.5 Temperature Control

Describes the temperature control system, range, and sensor used in the BioFlo 3000.

1.6 Aeration

Covers the aeration system, gas introduction, and flow rate control for the BioFlo 3000.

1.7 pH Control

Details the pH control system, range, electrode, and pump operation in the BioFlo 3000.

1.8 D.O. Control

Explains the Dissolved Oxygen control system, range, sensors, and control methods for the BioFlo 3000.

1.9 Foam Control

Describes the foam control mechanism using an antifoam probe and pump in the BioFlo 3000.

1.10 Exhaust System

Covers the exhaust system, including the condenser and filter for the BioFlo 3000.

1.11 Sampling System

Details the two systems available for collecting samples from the BioFlo 3000.

1.12 Specifications

Lists the technical specifications for the BioFlo 3000's vessel, temperature, agitation, exhaust, aeration, pH, and DO.

1.13 Operating Controls and Displays

Explains the membrane keypad, screen selection, and mode selection for operating the BioFlo 3000.

Chapter 2 - Installation

2.1 Inspection

Instructions for inspecting the BioFlo 3000 for damage after shipping and verifying components.

2.2 Utility Requirements

Lists the necessary water, gas, and electrical utility requirements for installing the BioFlo 3000.

2.3 Installation of Console

Provides steps for positioning, leveling, and connecting the BioFlo 3000 console.

2.4 Power Switch

Locates the main power switch and provides checks before turning on the system.

2.5 Recorder Output

Describes the recorder output pins and specifications for connecting an external recorder.

2.6 RS232;422 Computer Interface

Details the 25-pin "D" connector for computer interface and pin designations.

Chapter 3 - Preparation & Operation

3.1 Cleaning of Vessel

Step-by-step guide for cleaning the BioFlo 3000 vessel using detergent, water, and sterilization.

3.2 Vessel Assembly

Instructions for assembling the BioFlo 3000 vessel, including impellers, probes, and headplate.

3.3 pH Probe Preparation

Guidance on inspecting and preparing the pH electrode, including electrolyte level and air bubbles.

3.4 Installation of the pH Electrode

Describes the correct procedure for installing the pH electrode, including safety precautions.

3.5 Probe Maintenance and Storage

Provides instructions for maintaining and storing the pH probe, including filling solution and storage conditions.

3.6 pH Electrode Calibration

Details the step-by-step process for calibrating the pH electrode using buffer solutions.

3.7 Operation of Dissolved Oxygen (DO) Probe

Instructions for handling, sterilizing, and installing the DO probe for optimal performance.

3.8 Sterilization Procedure

Comprehensive guide for sterilizing the BioFlo 3000 vessel, components, and probes using an autoclave.

3.9 Dissolved Oxygen Electrode (D.O.) Calibration

Step-by-step methods for performing zero and span calibration for the D.O. electrode.

3.10 Preparation for Operation

Outlines the final steps to prepare the BioFlo 3000 system for operation after assembly and calibration.

3.11 D.O. Cascade System

Explains how the system controls D.O. by adjusting agitation speed and oxygen output.

3.12 Sampling Procedure

Provides detailed steps for collecting samples aseptically from the BioFlo 3000 vessel.

3.13 Shut-down Procedures

Outlines the correct steps for safely shutting down the BioFlo 3000 system.

3.14 Operation Tips

Offers practical advice for assembling the glass vessel, cleaning components, and calculating OTR.

Chapter 4 - Maintenance

4.1 General

Discusses the importance of preventive maintenance for equipment longevity and reducing downtime.

4.2 Console Cleaning

Provides instructions for cleaning the BioFlo 3000 console using a damp cloth and mild detergent.

4.3 Periodic Inspection

Details checks and inspections to be performed at three-month intervals to ensure proper operation.

4.4 Vessel and Tubing Cleaning

Specifies cleaning procedures for the vessel, headplate, and associated parts after each run.

4.5 Probe Maintenance and Storage

Guides on maintaining and storing D.O. and pH probes, including lifespan and storage conditions.

4.6 The Agitator Bearing Housing

Recommends checking and cleaning the agitator bearing housing components every 3-6 months.

4.7 Replacement Part List

Lists part numbers for various components of the BioFlo 3000 system.

Appendix: Cell Culture on BioFlo 3000

A. Vessel Assembly (Basic)

Lists the part numbers for basic vessel assemblies for different total volumes.

1. Fixed Bed Kit (Packed Bed Reactor)

Lists part numbers for the Fixed Bed Kit for different total volumes.

2. Cell Lift Impeller Kit

Lists part numbers for the Cell Lift Impeller Kit for different total volumes.

3. Marine Blade Impeller Kit

Lists part numbers for the Marine Blade Impeller Kit for different total volumes.

4. Pitched Blade Impeller Kit

Lists part numbers for the Pitched Blade Impeller Kit for different total volumes.

1. Probe kit for Cell Lift, Marine Blade and Pitched Blade Impeller Applications.

Lists part numbers for probe kits for specific impeller applications and total volumes.

2. Probe Kit for Fixed Bed (Packed Bed Reactor)

Lists part numbers for probe kits specifically for the Fixed Bed Reactor.

1. Perfusion Kit for Cell Lift Application.

Lists part numbers and voltage for Perfusion Kits for Cell Lift applications.

2. Perfusion Kit for Fixed Bed Application.

Lists part numbers and total volume for Perfusion Kits for Fixed Bed applications.

E. Conversion Kit M1217-0076.

Describes the contents of the Conversion Kit, including a CelliGen Plus manual and motor.

Summary

Chapter 1 - Introduction

1.1 Scope of Manual

Outlines the manual's content and scope for the BioFlo 3000 system.

1.2 Description of Equipment

Provides an overview of the BioFlo 3000 bioreactor system and its capabilities.

1.3 Description of Vessel

Details the construction and features of the BioFlo 3000's glass and stainless steel vessel.

1.4 Agitation System

Explains the servo motor, speed control, and PID loop for agitation in the BioFlo 3000.

1.5 Temperature Control

Describes the temperature control system, range, and sensor used in the BioFlo 3000.

1.6 Aeration

Covers the aeration system, gas introduction, and flow rate control for the BioFlo 3000.

1.7 pH Control

Details the pH control system, range, electrode, and pump operation in the BioFlo 3000.

1.8 D.O. Control

Explains the Dissolved Oxygen control system, range, sensors, and control methods for the BioFlo 3000.

1.9 Foam Control

Describes the foam control mechanism using an antifoam probe and pump in the BioFlo 3000.

1.10 Exhaust System

Covers the exhaust system, including the condenser and filter for the BioFlo 3000.

1.11 Sampling System

Details the two systems available for collecting samples from the BioFlo 3000.

1.12 Specifications

Lists the technical specifications for the BioFlo 3000's vessel, temperature, agitation, exhaust, aeration, pH, and DO.

1.13 Operating Controls and Displays

Explains the membrane keypad, screen selection, and mode selection for operating the BioFlo 3000.

Chapter 2 - Installation

2.1 Inspection

Instructions for inspecting the BioFlo 3000 for damage after shipping and verifying components.

2.2 Utility Requirements

Lists the necessary water, gas, and electrical utility requirements for installing the BioFlo 3000.

2.3 Installation of Console

Provides steps for positioning, leveling, and connecting the BioFlo 3000 console.

2.4 Power Switch

Locates the main power switch and provides checks before turning on the system.

2.5 Recorder Output

Describes the recorder output pins and specifications for connecting an external recorder.

2.6 RS232;422 Computer Interface

Details the 25-pin "D" connector for computer interface and pin designations.

Chapter 3 - Preparation & Operation

3.1 Cleaning of Vessel

Step-by-step guide for cleaning the BioFlo 3000 vessel using detergent, water, and sterilization.

3.2 Vessel Assembly

Instructions for assembling the BioFlo 3000 vessel, including impellers, probes, and headplate.

3.3 pH Probe Preparation

Guidance on inspecting and preparing the pH electrode, including electrolyte level and air bubbles.

3.4 Installation of the pH Electrode

Describes the correct procedure for installing the pH electrode, including safety precautions.

3.5 Probe Maintenance and Storage

Provides instructions for maintaining and storing the pH probe, including filling solution and storage conditions.

3.6 pH Electrode Calibration

Details the step-by-step process for calibrating the pH electrode using buffer solutions.

3.7 Operation of Dissolved Oxygen (DO) Probe

Instructions for handling, sterilizing, and installing the DO probe for optimal performance.

3.8 Sterilization Procedure

Comprehensive guide for sterilizing the BioFlo 3000 vessel, components, and probes using an autoclave.

3.9 Dissolved Oxygen Electrode (D.O.) Calibration

Step-by-step methods for performing zero and span calibration for the D.O. electrode.

3.10 Preparation for Operation

Outlines the final steps to prepare the BioFlo 3000 system for operation after assembly and calibration.

3.11 D.O. Cascade System

Explains how the system controls D.O. by adjusting agitation speed and oxygen output.

3.12 Sampling Procedure

Provides detailed steps for collecting samples aseptically from the BioFlo 3000 vessel.

3.13 Shut-down Procedures

Outlines the correct steps for safely shutting down the BioFlo 3000 system.

3.14 Operation Tips

Offers practical advice for assembling the glass vessel, cleaning components, and calculating OTR.

Chapter 4 - Maintenance

4.1 General

Discusses the importance of preventive maintenance for equipment longevity and reducing downtime.

4.2 Console Cleaning

Provides instructions for cleaning the BioFlo 3000 console using a damp cloth and mild detergent.

4.3 Periodic Inspection

Details checks and inspections to be performed at three-month intervals to ensure proper operation.

4.4 Vessel and Tubing Cleaning

Specifies cleaning procedures for the vessel, headplate, and associated parts after each run.

4.5 Probe Maintenance and Storage

Guides on maintaining and storing D.O. and pH probes, including lifespan and storage conditions.

4.6 The Agitator Bearing Housing

Recommends checking and cleaning the agitator bearing housing components every 3-6 months.

4.7 Replacement Part List

Lists part numbers for various components of the BioFlo 3000 system.

Appendix: Cell Culture on BioFlo 3000

A. Vessel Assembly (Basic)

Lists the part numbers for basic vessel assemblies for different total volumes.

1. Fixed Bed Kit (Packed Bed Reactor)

Lists part numbers for the Fixed Bed Kit for different total volumes.

2. Cell Lift Impeller Kit

Lists part numbers for the Cell Lift Impeller Kit for different total volumes.

3. Marine Blade Impeller Kit

Lists part numbers for the Marine Blade Impeller Kit for different total volumes.

4. Pitched Blade Impeller Kit

Lists part numbers for the Pitched Blade Impeller Kit for different total volumes.

1. Probe kit for Cell Lift, Marine Blade and Pitched Blade Impeller Applications.

Lists part numbers for probe kits for specific impeller applications and total volumes.

2. Probe Kit for Fixed Bed (Packed Bed Reactor)

Lists part numbers for probe kits specifically for the Fixed Bed Reactor.

1. Perfusion Kit for Cell Lift Application.

Lists part numbers and voltage for Perfusion Kits for Cell Lift applications.

2. Perfusion Kit for Fixed Bed Application.

Lists part numbers and total volume for Perfusion Kits for Fixed Bed applications.

E. Conversion Kit M1217-0076.

Describes the contents of the Conversion Kit, including a CelliGen Plus manual and motor.

Brunswick BIOFLO 3000 Specifications

General IconGeneral
BrandBrunswick
ModelBIOFLO 3000
CategoryLaboratory Equipment
LanguageEnglish

Related product manuals