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BIOFLO 3000
Brunswick BIOFLO 3000 User Manual
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BIOFLO 3000
BENCH-TOP
FERMENTOR
MANUAL NO.: M1217-0050/K
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 . 44 TALMADGE ROAD . EDISON, NJ 08818-4005 . 732-287-1200
TELEPHONE: 800-631-5417 . FAX: 732-287-4222 . TELEX: 4753012 NBSCO
INTERNET: http://www.nbsc.com/ . E-MAIL: bioinfo@nbsc.com
M1217-0050
1
Click here to view available Bioflo 3000 Bioreactor at
www.ssllc.com>>
2
Table of Contents
Table of Contents
3
Scope of Manual
4
Description of Equipment
4
Description of Vessel
4
Agitation System
4
Temperature Control
4
Aeration
4
Ph Control
5
D.o. Control
5
Foam Control
5
Exhaust System
5
Sampling System
5
Specifications
6
Operating Controls and Displays
7
Inspection
10
Utility Requirements
10
Installation of Console
10
Power Switch
10
Recorder Output
10
Computer Interface
11
Cleaning of Vessel
14
Vessel Assembly
14
Ph Probe Preparation
15
Installation of the Ph Electrode
15
Probe Maintenance and Storage
15
Ph Electrode Calibration
15
Operation of Dissolved Oxygen (Do) Probe
16
Sterilization Procedure
16
Dissolved Oxygen Electrode (D.O.) Calibration
18
Preparation for Operation
18
Cascade System
19
Sampling Procedure
20
Shut-Down Procedures
20
Operation Tips
21
General
23
Console Cleaning
23
Periodic Inspection
23
Vessel & Tubing Cleaning
23
Probe Maintenance & Storage
23
The Agitator Bearing Housing
24
Replacement Part List
24
Appendix Cell Culture on Bioflo 3000
26
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Brunswick BIOFLO 3000 Specifications
General
Brand
Brunswick
Model
BIOFLO 3000
Category
Laboratory Equipment
Language
English
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