Acid, Lvl 1, Lvl 2, Duty Cycle
Lvl 3, On Variable
Flowrates of Pumps
Flowrates (mL/min)* Silicone tubing only
Tubing internal diameter
0.5mm 0.8mm 1.6mm 3.2mm 4.8mm
Frequency RPM 1/50" 1/32" 1/16" 1/8" 3/16"
50 Hz 12 .252 .59 2.62 9.60 19.6
60 Hz 14.4 .300 .71 3.14 11.5 23.5
* NOTE: The numbers shown in the table are the flowrates when the control
mode of the feed pump is "ON". When the feed pump is set in other
modes (manual, base, acid, lvl 1, lvl 2 and lvl 3) the flowrates will
be 20% less than the numbers shown in the table.
1.13 OPERATING CONTROLS AND DISPLAYS
The main operating controls of the BioFlo 3000 is the membrane keypad. There are twenty
keys on the keypad. The function of "Screen" key is to select the screen. There are three
screens which can be selected on the display. The first one is the "Master" screen, the other
two are: a "Calibration" and a "Gases" screen (Fig. 12, 14, 15, 16).
Besides above three screens, there is a mode selection screen which displays only after
the power switch is turned on. The "Mode Selection" (Fig. 13) will display on the
screen for 20 seconds. Operator can select cell culture or fermentation by pressing "1"
or "2" within this period of time. Then the screen will automatically turn to the "Master
Screen".
MODE SELECTION
1. CELL CULTURE
2. FERMENTATION
Your Selection: 2
Figure 13
On the top of each selective screen, three screen names are listed, the one in brackets is the
screen currently displaying, the other two screens are to be selected. Press "Screen" key, the
middle one will blink, this means that this screen is ready to be selected, press "Enter" key,
the selection of a new screen is done. If another screen is to be selected, don't press "Enter"
key but arrow () key first, then press "Enter" key.
The function of the "Alter" key is to select the loop and the mode of each loop. When
M1217-0050 7
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