NOTE: pH electrode is calibrated before autoclaving vessel.
1. Connect electrode to the pH connector using the appropriate cable.
2. Turn ON the main power switch.
3. Display calibration screen.
NOTE: The pH measuring system is calibrated using two external buffer
solution of known pH.
4. Immerse pH electrode into pH 7.00 buffer solution and allow a few minutes for the
system to equilibrate.
5. Set the pH function zero.
6. Set the display to read 7.00.
7. Rinse the pH electrode with distilled water.
8. Immerse pH electrode into a second pH buffer solution which is several pH units
above or below pH 7.00 (eg. 4.00) and allow a few minutes for the system to
equilibrate.
9. Set the pH function "Span".
10. Set the display to read the value of the second buffer solution.
11. Repeat steps 5-10 using the same buffer solutions.
NOTE: The pH calibration should be checked after autoclaving immediately prior
to inoculation. This is performed by taking a sample from the vessel and
comparing the value of pH displayed on the screen with that of an external
pH meter. Any discrepancy should be adjusted with the function set to
"Zero".
3.7 OPERATION OF DISSOLVED OXYGEN (DO) PROBE
1. Remove protective cap from electrode end. The membrane is delicate and care must
be exercised to prevent accidental damage. Never rest probe on membrane.
2. To insure stable output, the probe should be subjected to two or three sterilization
(autoclaving) cycles prior to use. The probe will be operable after the second cycle
but will be more stable with additional sterilizations. The shorting plug should be
installed on the probe during autoclaving or sterilization.
3. Install probe into vessel head plate assembly (Fig. 6,7). If an Ingold probe is to be
used, wrap the adapter threads with teflon tape, screw into headplate and tighten
with a wrench. Carefully insert the probe into the adapter. Finger tighten enough
to compress the o-ring to insure a tight seal.
3.8 STERILIZATION PROCEDURE
Remove the motor drive from the top of the vessel and place it on the motor mount at the
top of the cabinet. Place the housing cover on the top of the bearing housing before
sterilization (Fig. 22).
Disconnect air line of the inlet filter side.
Disconnect all probes and remove probe cables.
Rubber T-stoppers should be in place in the pH probe during autoclaving. (Rubber
bands can be placed around them to prevent blow out during sterilization.)
Remove the sampler rubber bulb then insert glass wool into sampler port and close
M1217-0050 16
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