CHAPTER 3 - PREPARATION & OPERATION
(For preparation and operation of Cell Culture, please read CelliGen Plus Manual
M1176-0050 - included with conversion kit - see p. 23)
3.1 CLEANING OF VESSEL
A. Fill the vessel with a mild detergent and water solution. Let stand for one hour then
scour thoroughly. Use a brush on both inside and outside surfaces.
B. Drain the vessel and rinse several times with tap water. Repeat rinsing with distilled
water and let dry.
C. Sterilization (see 3.8).
3.2 VESSEL ASSEMBLY
A. Slide impellers on the shaft of the bearing housing and clamp them down. Lower
impeller should be positioned about 1/4 inch above the bottom of the baffle. Upper
impeller one to one and half impeller diameters above lower impeller (Fig. 18).
* Lubricate vessel O-ring with silicone grease, position glass tube reactor and
secure clamping screws, perform the same for head plate. DO NOT OVERTIGHTEN.
Working From the Inside of the Coverplate.
B. Insert the sparger tube in the sparger port, (Fig. 19).
C. Insert the harvest tube in the harvest port, (Fig. 20).
Working From the Outside of the Coverplate.
D. Insert the thermowell tube in the temperature control port, (Fig. 3).
E. Insert the sampler assembly into the sample port (Fig. 10).
F. Install foam probe in the head plate, (Fig. 8).
G. Install the baffle assembly inside of the glass jar by pressing the two edged baffles
together.
H. Install four polypropylene ports or plugs in the wall of the glass jar. Be sure to use
a blind plug (without hole) when a port is not in use, (Fig. 21).
Place head plate on the flange of the vessel and lock it to the clamping ring with
knurled screws (see operation tips). Install pH and DO probes (coat probe with
glycerin before insertion into head plate). Prior to installation, probes should be
well prepared (see sec. 3.3, 3.4, 3.5 and 3.7).
NOTE: To avoid damage to the probes make sure that no interference exists between
the probes and the baffle assembly.
Place the vessel on the three pins located on the base of the cabinet.
Position the motor assembly on top of the bearing housing locating pin. Connect the motor
cable to the receptacle on the face of the cabinet.
M1217-0050 14
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