C. Add glycerin to the thermowell and insert (RTD) temperature probe.
D. Turn on the water valve, adjust water pressure to 20 PSI.
E. Connect air and O2 to the unit, adjust gas pressure to 10 PSI.
CAUTION:
NEVER ATTACH TUBINGS MADE OF HARD MATERIAL, SUCH AS TEFLON, DIRECTLY
TO THE PLASTIC HOSE BARBS ON THE GAS ENTRY.
IN CASE HARD MATERIAL TUBING IS GOING TO BE USED, ATTACH A SHORT SOFT
TUBING (NBS P/N P0740-2430) BETWEEN THE HOSE BARB AND THE HARD TUBING.
F. Turn on the power switch.
G. Select fermentation mode by pressing key "2".
H. On master screen, set temp loop control mode to "Prime" for 1 minute.
I. Set agitation to desired speed and then set mode to PID.
J. Set temp to the desired working temperature set mode to PID.
K. Remove plugs and tape (if any) and shorting cap from the pH probe and connect the
pH cable to the pH probe.
L. Remove the protecting cap from the D.O. probe and connect the D.O. cable to the
D.O. probe.
M. When the vessel reaches desired working temperature, calibrate the D.O. probe.
N. Set pH and D.O. to the desired set points and set pH and D.O. mode to PID.
O. Set agitation to D.O. if applying D.O. cascade system (see 3.11).
P. Set 2-Gas mode to D.O. or AgO2DO, if applying oxygen enrichment/D.O. cascade
system (see 3.11.2). Otherwise, set 2-Gas mode to manual.
Q. Adjust air flow rate to the desired value.
3.11 D.O. CASCADE SYSTEM
The system is designed to control D.O. by PID control of agitation speed and oxygen
output. When D.O. actual value is above the D.O. setpoint, the agitation speed will
automatically decrease until the D.O. setpoint is reached.
As the D.O. level drops below the setpoint, there are three ways to pick it up.
M1217-0050 19
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