TABLE OF CONTENTS
INTRODUCTION
1.1 Scope of Manual
1.2 Description of Equipment
1.3 Description of Vessel
1.4 Agitation system
1.5 Temperature Control
1.6 Aeration
1.7 pH Control
1.8 D.O. Control
1.9 Foam Control
1.10 Exhaust System
1.11 Sampling System
1.12 Specifications
1.13 Operating Controls and Displays
INSTALLATION
2.1 Inspection
2.2 Utility Requirements
2.3 Installation of Console
2.4 Power Switch
2.5 Recorder Output
2.6 Computer Interface
PREPARATION AND OPERATION
3.1 Cleaning of Vessel
3.2 Vessel Assembly
3.3 pH Probe Preparation
3.4 Installation of the pH Electrode
3.5 Probe Maintenance and Storage
3.6 pH Electrode Calibration
3.7 Operation of Dissolved Oxygen (D.O.) Probe
3.8 Sterilization Procedure
3.9 Dissolved Oxygen Electrode (D.O.) Calibration
3.10 Preparation for Operation
3.11 D.O. Cascade System
3.12 Sampling Procedure
3.13 Shut-Down Procedures
3.14 Operation Tips
MAINTENANCE
4.1 General
4.2 Console Cleaning
4.3 Periodic Inspection
4.4 Vessel & Tubing Cleaning
4.5 Probe Maintenance & Storage
4.6 The Agitator Bearing Housing
4.7 Replacement Part List
APPENDIX: Cell Culture on BioFlo 3000
LIST OF DRAWINGS
M1217-0050 3