If after autoclaving, most of the liquid has exhausted from the vessel, the autoclave is
exhausting too quickly. Adjust the autoclave to exhaust more slowly. Attach a piece
of tubing with some non-absorbent material such as glass wool or non-absorbent
cotton and some foil wrapped on the ends to one of the addition ports. This helps the
vessel to vent more easily during autoclaving. Immediately crimp the foil and close
off the vent tubing to maintain sterility.
3.9 DISSOLVED OXYGEN ELECTRODE (D.O.) CALIBRATION
NOTE: D.O. electrode is calibrated after autoclaving vessel.
IMPORTANT NOTE:
When the system is operated for the first time, or when the electrode has been
disconnected from the voltage source (amplifier or polarization module) for
longer than 5 to 10 minutes, the electrode must be connected to the operating
O2 amplifier for polarization purpose prior to calibration.
The electrode is polarized and ready for operation after six hours of
polarization time.
1. There are two methods of obtaining zero on the BioFlo 3000. Use either Method 1
or Method 2.
Method 1
a. Remove the D.O. electrode cable from the D.O. electrode.
b. Display calibration screen.
c. Set the D.O. function to "Zero".
d. Set the display to read zero by setting "Zero" to 0.
e. Re-connect the D.O. electrode cable to the D.O. electrode.
Method 2
a. Connect the D.O. electrode cable to the D.O. electrode.
b. Set agitation speed to 500 RPM.
c. Sparge nitrogen into the vessel via the filter on the head plate until the D.O.
display is stable for approximately 10 minutes (this may take up to 30
minutes).
d. Display calibration screen.
e. Set the function to "Zero".
f. Set the display to read zero by setting "Zero" to 0.
2. Setting the "Span".
a. Set the agitation rate of 500 RPM and agitation mode PID.
b. Vigorously sparge air or oxygen into the vessel via the filter on the head plate
until the display is stable for approximately 10 minutes (this may take up to
30 minutes).
c. Set the function to "Span".
d. Set the display to read 100 by setting "Span" to 100.
3.10 PREPARATION FOR OPERATION
A. Position and secure the vessel on BioFlo 3000 console. Connect the heat exchanger
and exhaust condenser.
B. Carefully place the DC servo motor on the vessel assembly.
M1217-0050 18
To Next Page
Loading...