sampler valve.
Sterilize the complete assembly consisting of jar, headplate and components of
headplate by inserting in an autoclave.
NOTE: For continuous culture, vessel should not be sterilized empty. Use at least
100 mL of sterilized water. Probe tips must be moist during sterilization.
After autoclaving medium will be brought from the medium reservoir,
sterilized separately. For batch fermentation the medium is sterilized
with the vessel.
*Autoclave at a temperature of 121C at 15 PSIG for 25 minutes.
CAUTION:
WHEN AUTOCLAVING THE GLASS VESSEL. IT MUST BE VENTED AT ALL TIMES.
DURING STERILIZATION OF THE GLASS REACTOR VESSEL, CONTENTS, AND
ELECTRODES IT IS IMPORTANT THAT THE PRESSURE BUILT UP IN THE AUTOCLAVE IS
RELEASED ONLY WHEN TEMPERATURE HAS DROPPED BELOW 90C. SLOW EXHAUST
IS REQUIRED.
CAUTION:
AT NO TIME SHOULD YOU SUBJECT THE PROBE TO CONDITIONS WHICH WOULD
CAUSE THE ELECTROLYTE TO BOIL. PARTICULAR CARE SHOULD BE EXERCISED
DURING THE COOLING CYCLE FOLLOWING AUTOCLAVING WHEN LOCALIZED
BOILING TENDS TO OCCUR. EITHER MAINTAIN A SLOW COOLING RATE OR
PRESSURIZE THE AUTOCLAVE DURING THIS PERIOD. CONSULT YOUR AUTOCLAVE
MANUFACTURER FOR INFORMATION REGARDING A PRESSURE BALANCING FEATURE
AND PROPER AUTOCLAVING TECHNIQUE TO ELIMINATE LOCALIZED BOILING
DURING THE COOLING CYCLE.
M1217-0050 17
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