3.11.1 Agitation/D.O. Cascade System
To set up, proceed as follows:
a. Set agitation and D.O. loop to PID mode.
b. Set agitation to the minimum RPM you wish for D.O. control.
c. Change agitation mode to D.O..
d. Set agitation speed to maximum RPM you wish for D.O. control.
e. D.O. is now controlled by regulation of the agitation speed between the limits
that have been set (minimum/maximum setpoints).
3.11.2 Oxygen Enrichment/D.O. Cascade System
To setup, proceed as follows:
a. Set D.O. loop to PID mode.
b. Set 2-Gas to D.O. mode.
c. Select proper PI value*
d. D.O. is now controlled by regulation of the percentage of oxygen output between
0 and 100.
* NOTE: P.I. values have been set in the factory before shipping. Under normal
conditions, it is not necessary to reset these values.
3.11.3 Agitation/Oxygen/D.O. Cascade System
To setup, proceed as follows:
a. Set agitation and D.O. loop to PID mode.
b. Set agitation to the minimum RPM you wish for D.O. control.
c. Change agitation mode to D.O.
d. Set agitation speed to maximum RPM you wish for D.O. control.
e. Set 2-Gas to "AgO2DO" mode.
f. Select proper PI value (see note of 3.11.2.C).
g. D.O. is now controlled by the regulation of agitation speed first. After the maximum
set speed is reached, then D.O. is controlled by the regulation of the percentage of
oxygen output.
3.12 SAMPLING PROCEDURE
To sample the culture proceed as follows: (Fig. 10)
1. Check to be sure that the sample bottle is loose not sealed against sampler gasket.
2. Close the valve on the sampler tube, if open.
3. Squeeze the bulb and then see that sample bottle is sealed against sampler gasket.
Open valve and obtain desired volume of sample. Close valve.
4. Remove bottle from sampler. Place the cap from a new bottle on the bottle containing
sample and install the new bottle in the sampler and make sure that the sample bottle
is firmly sealed against the sampler gasket. Use aseptic techniques.
5. Repeat step 1 through 4 until desired number of samples are taken.
3.13 SHUT-DOWN PROCEDURES
To shut-down the system proceed as follows:
1. Shut-off gas flow.
M1217-0050 20
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