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- V Series Manual Fill Instructions -
The unique design of the new Isothermal Storage Unit is such that it is no longer necessary
to fill the storage chamber with liquid nitrogen. Instead, the inner walls of the storage vessel
are filled with liquid nitrogen and the storage chamber holds no liquid at all. Because of this
unique design the manual filling of this equipment is very different from storage units of the
past. Use the following instructions to manually fill the Isothermal Storage Unit in the event
of a power outage or if the autofill is not working properly.
For correct manual filling of the Isothermal unit perform the following steps:
1) Turn off the liquid nitrogen supply valve at the supply tank.
2) Disconnect the supply line from the Isothermal Unit at the port on the side of the unit.
3) Remove the cap from the manual fill port, on the left side of the unit.
4) Place the cap on the autofill port where the supply line was connected, and tighten down.
5) Connect the liquid nitrogen supply line to the manual fill port, and tighten down.
Without power there will be no digital display to give the liquid level, and the liquid can-
not be seen visually since it is contained inside the walls of the Isothermal Unit. With this in
mind, there are two ways to gauge the liquid level when manually filling the Unit.
The first method is to watch the coloration of the inside of the storage vessel as the liquid
is filling the walls. There will be a slight variation in the color of the tank walls at the liquid
level as it moves up inside the walls. The walls of the vessel will look somewhat "wet" below
the liquid level line. It may be necessary to look carefully to perceive this change in the look
of the tank walls, but it will be there. Remember, however, not to place your head down into
the vessel when watching for this change, as this may be hazardous to your health.
The second way to gauge the fill level is the last resort, because it is only noticeable when
the vessel is nearly full. When the tank is nearing maximum fill, liquid nitrogen will begin to
"spatter" through the vents located near the top edge of the inner walls of the vessel. When
this is seen, the supply valve should be shut off immediately before the liquid nitrogen
begins pouring into the vessel, creating potentially adverse conditions.
Check unit daily to ensure proper operation and safety of the stored samples. For the V Series
units it is essential to lift the lid each day and check for vapor and signs of proper freezing.
IMPORTANT: NEVER attempt to manually fill the Isothermal Storage Unit by opening the lid
and placing a fill line over the edge into the vessel. This is an incor
rect fill method and may
cause damage to the stored samples.
CAUTION: Before opening the supply valve to fill the Isothermal Unit, read the following
text to learn how to gauge the liquid level, so the Storage Unit does not overfill and create
hazardous conditions.
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