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cytiva Xuri W25 User Manual

cytiva Xuri W25
290 pages
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Xuri
Cell Expansion System
W25
User Manual
cytiva.com

Table of Contents

Other manuals for cytiva Xuri W25

Questions and Answers:

cytiva Xuri W25 Specifications

General IconGeneral
ModelXuri W25
TypeCell Culture System
Cell Culture Capacityup to 25 L
Working Volume Range5-25 L
pH ControlYes
Dissolved Oxygen ControlYes
Agitation TypeRocking
SterilizationGamma-irradiated, ready-to-use
MaterialSingle-use Cellbag bioreactor
DescriptionThe Xuri W25 is a single-use cell expansion system designed for adherent and suspension cell culture.

Summary

1 Introduction

1.1 Important User Information

Covers critical safety instructions, intended use, prerequisites, and safety notices for users.

1.3 Associated Documentation

Lists related documents for the Xuri Cell Expansion System W25, including operating instructions and guides.

2 System Description

2.1 System Overview

Provides a general overview of the Xuri Cell Expansion System W25 and its intended use for cell cultivation.

2.2 Xuri Cell Expansion System W25 Rocker

Details the rocker as the main system unit, its functions, and rocking parameters.

2.3 Xuri Cell Expansion W25 CBCU

Introduces the CBCU control unit and its configurations, showing front panel components.

2.5 Cellbag Bioreactor

Describes the Cellbag bioreactor, its options, and shows an illustration of its components.

2.6 UNICORN Software Overview

Gives an overview of the UNICORN software and its help utility, listing sections within.

3 The UNICORN Software

3.2 System Control

Details the System Control module for starting, viewing, and controlling runs, including its panes.

3.3 Methods in UNICORN

Covers creating and working with methods and text instructions in UNICORN.

4 System Control Description

4.3 pH and DO Measurement and Control

Introduces the principles of pH and DO measurement and control in the Xuri Cell Expansion System W25.

4.6 Media Control

Covers media control modes (Media addition, Perfusion) and references for settings.

5 Operation

5.1 Set Up the System

Guides on preparing the bioreactor system for cell cultivation, referring to system description.

5.2 Start and Configure the System

Guides on starting the system, logging into UNICORN, connecting, and configuring the system in software.

5.3 Prepare for Cultivation

Covers preparing the system for cell cultivation, including bioreactor and sensor preparation.

5.4 Perform Cultivation

Describes the basics of performing a cultivation, monitoring parameters and adjusting settings.

6 Maintenance

6.1 Calibration

Outlines the calibration schedule before cultivation and when required, including scale and sensor calibrations.

6.2 Pump Calibration

Explains pump calibration procedures, tube inner diameter importance, and rate modes.

7 Troubleshooting

7.2 Xuri Cell Expansion System W25 Rocker

Addresses troubleshooting for rocker issues like initialization, rocking, angle, speed, and power button errors.

7.3 Xuri Cell Expansion W25 CBCU

Covers general CBCU error symptoms, gas flow issues, and CO2/O2 mix problems.

7.6 Software Problems

Introduces troubleshooting for UNICORN software issues, listing specific modules.

8 Reference Information

8.1 System Specifications

Lists the overall system specifications for Xuri Cell Expansion System W25, including configuration and supply requirements.

8.2 Component Specifications

Details equipment dimensions, weights, and specific specifications for the Rocker unit.

8.5 Control Settings

States this section lists various control settings configurable in UNICORN.

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