cytiva AKTA ready User Manual (English)

Questions and Answers:

J
James MartinezAug 20, 2025
What to do if there is air in the flow path in cytiva AKTA ready?
- A
  andrewwolfeAug 21, 2025
  If you find air in the flow path, identify the cause. This could be due to damaged tubing or a connector, a dislocated TC gasket, or the inlet tubing not being properly located in the liquid. In rare instances, cavitation from negative pressure might be the cause. Locate and fix the issue, checking the entire flow path, including the inlet containers. Additionally, you can use the ManFlow command in UNICORN to remove air from the flow path. To proceed with the run, click 'Continue' on the toolbar.
M
Mark AdamsAug 22, 2025
What to do if little or no flow is detected in cytiva AKTA ready?
- S
  samanthawarrenAug 22, 2025
  If you observe little or no flow, check if the connected inlet is closed, and ensure the containers are not placed lower than 1 meter below the inlets. Verify that the inlet containers are not empty. Inspect the flow kit tubing for wear; if worn, replace the flow kit. Also, check for any kinks or squeezes in the tubing that might be blocking the flow; reinstall or replace the flow kit if necessary. If there's air in the pump tubing, increase the pump speed to push the air to the air trap.
S
Shannon HenryAug 25, 2025
What causes pulsation of liquid in flow kit of cytiva AKTA ready?
- C
  Carla FarmerAug 25, 2025
  Pulsation of liquid in the flow kit can occur if the viscosity of the liquid is not within the specified range. Check that the viscosity of liquid is within specified range. Also, check if the pump speed is high enough and increase it if necessary.
C
christopherdavisAug 26, 2025
What to do if the pump is not working in cytiva AKTA ready Laboratory Equipment?
- G
  griffithseanAug 26, 2025
  If the pump isn't working, first check if the EMERGENCY STOP button has been pressed. Ensure the pump lid is correctly attached and closed. Verify that a valid flow path is defined, with at least one inlet and one outlet open, either in UNICORN or on the system itself. Also, check if the method being used lacks a pump flow instruction, and verify the pump function.
A
Anne StevensonAug 28, 2025
What to do if compressed air problem occurs in cytiva AKTA ready?
- M
  Michael GuerreroAug 28, 2025
  If you encounter compressed air issues, identify the cause, such as a malfunctioning air supply or a leaking air hose. Ensure the supply pressure is above 5.5 bar and correct any air supply problems. You can continue the run by pressing the PAUSE/CONTINUE button on the system cabinet.
C
Christine KingAug 29, 2025
What to do if the message Fine tuning flow meter failed is displayed on cytiva Laboratory Equipment?
- J
  Jennifer HillAug 29, 2025
  If the message 'Fine tuning flow meter failed' appears, it indicates that there is not enough lubricant on the transducers. Apply more lubricant as described in the manual section 'Apply lubricant to flow meter'. If you are using buffers with a high salt concentration, you might want to disable the message.
S
Susan JonesAug 31, 2025
What causes drifting UV signal in cytiva AKTA ready Laboratory Equipment?
- M
  Matthew DavidsonAug 31, 2025
  A drifting UV signal can be caused by large temperature differences leading to condensation on the UV sensor. Ensure that the temperature of the process liquid is not more than 10°C below the ambient temperature. If necessary, wipe the UV sensor with a soft tissue to remove any condensation.
J
Jennifer MillerSep 2, 2025
What to do if overpressure is detected in cytiva Laboratory Equipment?
- W
  William MillerSep 2, 2025
  If overpressure is detected and the air trap is in-line, press the AIR VENT button on the system cabinet to reduce the pressure, as overpressure can damage the flow kit. If overpressure is detected when the air trap is bypassed, set Manual Flow to 0 in UNICORN and set the air trap in-line. Click CONTINUE in UNICORN, or PAUSE/CONTINUE on the system cabinet. Press the AIR VENT button on the system cabinet until the overpressure is released.
E
Edwin MitchellSep 4, 2025
What to do if cavitation occurs in cytiva Laboratory Equipment?
- S
  Shannon EvansSep 4, 2025
  If you're experiencing cavitation, which results in unstable flow meter readings, check the flow path for kinks and inspect the inlet containers. Make sure the inlet tubing diameter is equal to or larger than the inlet manifold tubing diameter. You can also remove any air in the flow path using the ManFlow command in UNICORN. To continue the run, click 'Continue' on the toolbar.
R
Rebecca GonzalesSep 5, 2025
What happens if there is a power failure during a run on cytiva AKTA ready?
- M
  Melissa Hughes MDSep 5, 2025
  In the event of a power failure during a run, check the system circuit breaker and the external power supply.

cytiva AKTA ready Specifications

General

Intended Use	Protein purification
Conductivity Monitoring	Yes
pH Monitoring	Yes
Fraction Collection	Yes
Software	UNICORN
Flow Rate Range	0.001-100 mL/min
UV Wavelengths	280 nm
Power Requirements	100-240 V, 50/60 Hz

Summary

Introduction

Important User Information

Key safety and operational information for all users of ÄKTA ready.

About this Manual

Details the purpose, scope, and typographical conventions used in this manual.

Associated Documentation

Lists related literature and resources available for ÄKTA ready and flow kits.

Abbreviations

Defines abbreviations used in the user documentation for ÄKTA ready.

Safety Instructions

Safety Precautions

Covers general and specific safety precautions for operating the ÄKTA ready system.

Labels

Describes the system and safety labels attached to the product.

Emergency Procedures

Details procedures for emergency shutdown and restarting the system.

System Description

Overview

Provides a general description of the ÄKTA ready system's features and purpose.

System Illustrations

Illustrates ÄKTA ready with flow kits, showing key components and their labels.

System Components

Details the various components that make up the ÄKTA ready system.

Flowchart

Presents a schematic diagram of the ÄKTA ready flow path, including color coding.

Flow Kits

Describes the disposable flow kits and their components for isocratic and gradient operations.

UNICORN Control System

Explains the role of UNICORN software in controlling the ÄKTA ready system.

Installation

Safety Precautions

Details safety precautions to be followed during the installation of ÄKTA ready.

Site Requirements

Outlines the environmental and physical requirements for the installation site.

Included in the Delivery

Lists the components and accessory kit included with the ÄKTA ready system.

Unpacking

Provides instructions on how to safely unpack the ÄKTA ready system and flow kit.

Transport

Covers safe procedures for moving, lifting, and transporting the ÄKTA ready system.

Connections

Guides on how to connect power, compressed air, computer, and external equipment.

Finish Installation

Describes steps to establish computer connection and equilibrate the system to temperature.

Operation

Safety Precautions

Covers critical safety precautions for operating the ÄKTA ready system.

Start the System and Log On to UNICORN

Guides on starting the ÄKTA ready system and logging into the UNICORN software.

Install Flow Kit

Step-by-step instructions for installing the isocratic and gradient flow kits.

Perform a Component Test

Instructions for running and understanding the results of component tests.

Connect a Column

Details on installing columns, performing efficiency tests, and rinsing.

Set ÄKTA Ready Specific Parameters in Method Editor

How to configure ÄKTA ready specific settings within the UNICORN Method Editor.

Run a Method

Procedures for preparing, starting, monitoring, and ending a method run.

Controlled Shutdown

Instructions for safely removing flow kits/columns and shutting down the system.

Maintenance

Safety Precautions

Essential safety precautions for performing maintenance on the ÄKTA ready system.

Maintenance Schedule

Provides a schedule for daily and annual maintenance tasks.

Maintenance Procedures

Detailed procedures for common maintenance tasks like cleaning and calibration.

Calibration

Instructions for calibrating the system's sensors and gradient pump.

Cleaning

Procedures for cleaning the ÄKTA ready system to ensure proper function.

Storage

Guidelines for preparing the system for storage.

Troubleshooting

Troubleshooting the System

Identifies and provides corrective actions for common system issues.

Troubleshooting a Failed Component Test

Guidance for resolving issues when a component test fails.

Reference Information

Specifications

Provides technical specifications for ÄKTA ready system capacity and pressure ratings.

Chemical Resistance

Lists recommended cleaning agents and allowed chemicals for ÄKTA ready.

Environmental Requirements

Specifies the environmental conditions required for operating and storing the system.

PID Control

Explains PID control principles and parameters used in ÄKTA ready.

Recycling Information

Information regarding product decommissioning, decontamination, and disposal.

Regulatory Information

Lists applicable regulations and standards for the ÄKTA ready system.

Flow Kit Ordering Information

Provides product codes and specifications for ordering flow kits.

More Information

Details on spare parts, accessories, contact information, and training.

Health and Safety Declaration Form

A form for declaring the safety status of equipment prior to service.

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cytiva AKTA ready User Manual

Table of Contents

Questions and Answers:

cytiva AKTA ready Specifications

Summary

Introduction

Important User Information

About this Manual

Associated Documentation

Abbreviations

Safety Instructions

Safety Precautions

Labels

Emergency Procedures

System Description

Overview

System Illustrations

System Components

Flowchart

Flow Kits

UNICORN Control System

Installation

Safety Precautions

Site Requirements

Included in the Delivery

Unpacking

Transport

Connections

Finish Installation

Operation

Safety Precautions

Start the System and Log On to UNICORN

Install Flow Kit

Perform a Component Test

Connect a Column

Set ÄKTA Ready Specific Parameters in Method Editor

Run a Method

Controlled Shutdown

Maintenance

Safety Precautions

Maintenance Schedule

Maintenance Procedures

Calibration

Cleaning

Storage

Troubleshooting

Troubleshooting the System

Troubleshooting a Failed Component Test

Reference Information

Specifications

Chemical Resistance

Environmental Requirements

PID Control

Recycling Information

Regulatory Information

Flow Kit Ordering Information

More Information

Health and Safety Declaration Form

Related product manuals