cytiva AKTA ready User Manual

ÄKTA
ready
Operating Instructions
Original instructions
cytiva.com

cytiva AKTA ready Specifications

General

Intended Use	Protein purification
Conductivity Monitoring	Yes
pH Monitoring	Yes
Fraction Collection	Yes
Software	UNICORN
Flow Rate Range	0.001-100 mL/min
UV Wavelengths	280 nm
Power Requirements	100-240 V, 50/60 Hz

Summary

Introduction

Important User Information

Key safety and operational information for all users of ÄKTA ready.

About this Manual

Details the purpose, scope, and typographical conventions used in this manual.

Associated Documentation

Lists related literature and resources available for ÄKTA ready and flow kits.

Abbreviations

Defines abbreviations used in the user documentation for ÄKTA ready.

Safety Instructions

Safety Precautions

Covers general and specific safety precautions for operating the ÄKTA ready system.

Labels

Describes the system and safety labels attached to the product.

Emergency Procedures

Details procedures for emergency shutdown and restarting the system.

System Description

Overview

Provides a general description of the ÄKTA ready system's features and purpose.

System Illustrations

Illustrates ÄKTA ready with flow kits, showing key components and their labels.

System Components

Details the various components that make up the ÄKTA ready system.

Flowchart

Presents a schematic diagram of the ÄKTA ready flow path, including color coding.

Flow Kits

Describes the disposable flow kits and their components for isocratic and gradient operations.

UNICORN Control System

Explains the role of UNICORN software in controlling the ÄKTA ready system.

Installation

Safety Precautions

Details safety precautions to be followed during the installation of ÄKTA ready.

Site Requirements

Outlines the environmental and physical requirements for the installation site.

Included in the Delivery

Lists the components and accessory kit included with the ÄKTA ready system.

Unpacking

Provides instructions on how to safely unpack the ÄKTA ready system and flow kit.

Transport

Covers safe procedures for moving, lifting, and transporting the ÄKTA ready system.

Connections

Guides on how to connect power, compressed air, computer, and external equipment.

Finish Installation

Describes steps to establish computer connection and equilibrate the system to temperature.

Operation

Safety Precautions

Covers critical safety precautions for operating the ÄKTA ready system.

Start the System and Log On to UNICORN

Guides on starting the ÄKTA ready system and logging into the UNICORN software.

Install Flow Kit

Step-by-step instructions for installing the isocratic and gradient flow kits.

Perform a Component Test

Instructions for running and understanding the results of component tests.

Connect a Column

Details on installing columns, performing efficiency tests, and rinsing.

Set ÄKTA Ready Specific Parameters in Method Editor

How to configure ÄKTA ready specific settings within the UNICORN Method Editor.

Run a Method

Procedures for preparing, starting, monitoring, and ending a method run.

Controlled Shutdown

Instructions for safely removing flow kits/columns and shutting down the system.

Maintenance

Safety Precautions

Essential safety precautions for performing maintenance on the ÄKTA ready system.

Maintenance Schedule

Provides a schedule for daily and annual maintenance tasks.

Maintenance Procedures

Detailed procedures for common maintenance tasks like cleaning and calibration.

Calibration

Instructions for calibrating the system's sensors and gradient pump.

Cleaning

Procedures for cleaning the ÄKTA ready system to ensure proper function.

Storage

Guidelines for preparing the system for storage.

Troubleshooting

Troubleshooting the System

Identifies and provides corrective actions for common system issues.

Troubleshooting a Failed Component Test

Guidance for resolving issues when a component test fails.

Reference Information

Specifications

Provides technical specifications for ÄKTA ready system capacity and pressure ratings.

Chemical Resistance

Lists recommended cleaning agents and allowed chemicals for ÄKTA ready.

Environmental Requirements

Specifies the environmental conditions required for operating and storing the system.

PID Control

Explains PID control principles and parameters used in ÄKTA ready.

Recycling Information

Information regarding product decommissioning, decontamination, and disposal.

Regulatory Information

Lists applicable regulations and standards for the ÄKTA ready system.

Flow Kit Ordering Information

Provides product codes and specifications for ordering flow kits.

More Information

Details on spare parts, accessories, contact information, and training.

Health and Safety Declaration Form

A form for declaring the safety status of equipment prior to service.